Effect of incubation with lipopolysaccharide and interferon- γ on reactive astrogliosis.

Effect of incubation with lipopolysaccharide and interferon-γ on reactive astrogliosis. J Integr Neurosci. 2019 Dec 30;18(4):415-421 Authors: Hua X, Wang Y, Yang C, Chai R, Li X, Tayier G, Pan P, Yu X Abstract Sepsis associated encephalopathy is a common complication of sepsis, but its pathogenesis of sepsis-associated encephalopathy remains unclear. Astrocytes are the most abundant brain glial cells, and reactive astrogliosis, a pathological response to central nervous system diseases, has a clear disease and disease-stage specificity. Functional changes of astrocytes are of great significance for the detection and prognosis of sepsis-associated encephalopathy. The pathogenesis of sepsis-associated encephalopathy was explored at the cellular level by examining astrogliosis in an in vitro model of sepsis-associated encephalopathy. Astrocytes of Wistar neonatal rats were incubated with different concentrations of lipopolysaccharide combined with interferon-γ. Cell viability was assessed by levels of tumor necrosis factor-α, interleukin-6, nitric oxide, reactive oxygen species, glial fibrillary acidic protein, changes of astrocyte morphology, and prevalence of apoptosis and necrosis. Compared with the control group, the cell viability of treated groups was decreased. The levels of tumor necrosis factor-α, interleukin-6, nitric oxide, reactive oxygen species, and glial fibrillary acidic protein were increased, hypertrophy of astrocy...
Source: Journal of Integrative Neuroscience - Category: Neuroscience Authors: Tags: J Integr Neurosci Source Type: research