Mitochondrial angiotensin II receptors regulate oxygen consumption in kidney mitochondria from healthy and type 1 diabetic rats.

Mitochondrial angiotensin II receptors regulate oxygen consumption in kidney mitochondria from healthy and type 1 diabetic rats. Am J Physiol Renal Physiol. 2020 Jan 06;: Authors: Friederich-Persson M, Persson P Abstract Exaggerated activation of the renin angiotensin aldosterone system (RAAS) is a key feature in diseases such as hypertension, diabetes and chronic kidney disease. Recently, an intracellular RAAS was demonstrated with angiotensin II (Ang II) type 1 and type 2 receptors (AT1 receptor and AT2 receptor) expressed in nuclei and mitochondria. Diabetes is associated with both mitochondrial dysfunction and increased intracellular Ang II concentration in the kidney cortex. The present study investigated the role of Ang II signaling in kidney cortex mitochondria isolated from control and streptozotocin-induced diabetic rats. Mitochondrial oxygen consumption was evaluated after addition of Ang II alone or after pre-incubation with candesartan (AT1 receptor antagonist), PD-123319 (AT2 receptor antagonist) or the two in combination. Ang II binds to only mitochondrial AT2 receptors in control rats and both AT1 receptors and AT2 receptors in diabetic rats. Ang II decreased oxygen consumption in mitochondria from both control and diabetic rats. Ang II response was reversed to increased oxygen consumption by nitric oxide synthase inhibitor L-NAME. AT1 receptor inhibition did not affect the response to Ang II, whereas AT2 receptor inhi...
Source: Am J Physiol Renal P... - Category: Urology & Nephrology Authors: Tags: Am J Physiol Renal Physiol Source Type: research