Isolation, culture, characterization, and adipogenic differentiation of heifer endometrial mesenchymal stem cells

Abstract Mesenchymal stem cells (MSCs) have been isolated from various tissues of different species. The mammalian endometrium has morphological and functional modifications throughout the estrous cycle undergoing periodic proliferation and degeneration. The aim of this study was to isolate, culture, and characterize and to determine adipogenic differentiation of endometrial mesenchymal stem cells (En-MSCs) in heifers. Uteri of healthy heifers were collected from Shiraz Slaughterhouse, Iran and transferred to Stem Cell Laboratory of Stem Cell and Transgenic Technology Research Center to isolate En-MSCs from endometrial tissue samples. The tissue samples were exposed to collagenase type IA to reach the primary culture of En-MSCs. Isolated En-MSCs were sub-cultured up to passage 4. A specified number of En-MSCs were seeded into 12- and 24-well culture plates, and the number of cells was counted to evaluate the growth behavior of isolated cells and the population doubling time (PDT). RT-PCR for CD45 marker (hematopoietic stem cells) and CD73 marker (MSCs) and differentiation to adipocytes were performed for MSCs confirmation of En-MSCs of heifer. After cell culture, spindle-shape En-MSCs were visible adherent to the culture flasks. The cell count and the growth curves using 12- and 24-well culture plates showed that the PDT of En-MSCs was 37, 159.5, 52.9, and 136.3 h after seeding 2.2 × 104 and 20 × 104 (12-well) and 5&thinsp...
Source: Comparative Clinical Pathology - Category: Pathology Source Type: research

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CONCLUSION: This study found that childhood cancer survivors in New Zealand had a high prevalence of developmental dental abnormalities and it identified potential risk factors related to their cancer treatment. Inequitable access to oral rehabilitation for this patient group argues for a mechanism for consistent improved access to publicly funded dental care across district health boards in New Zealand. PMID: 33032302 [PubMed - in process]
Source: New Zealand Medical Journal - Category: General Medicine Tags: N Z Med J Source Type: research
Publication date: Available online 10 October 2020Source: Hematology/Oncology and Stem Cell TherapyAuthor(s): Thejus T. Jayakrishnan, Veli Bakalov, Zena Chahine, John Lister, Rodney E. Wegner, Santhosh Sadashiv
Source: Hematology Oncology and Stem Cell Therapy - Category: Cancer & Oncology Source Type: research
Publication date: 15 February 2021Source: Chemical Engineering Journal, Volume 406Author(s): Do Kyung Kim, Sanghyuk Lee, Minjeong Kim, Yunhui Jeong, Soonchul Lee
Source: Chemical Engineering Journal - Category: Chemistry Source Type: research
AbstractLike other malignancies, prostate tumors are thought to contain cancer stem-like cells (CSCs) that are responsible for growth, metastasis, and therapy resistance. ΔNp63 (also called p40) is a regulator of normal prostate stem/progenitor cell activities and a marker of normal basal epithelial cells. The levels of ΔNp63 are reduced in prostate adenocarcinomas, although there is also evidence that ΔNp63 is involved in CSC regulation and drives metastasis to t he bone. We studied metastatic deposits of prostate cancers with isoform-specific ΔNp63 and TAp63 antibodies. We identified p63-positive ...
Source: Virchows Archiv - Category: Pathology Source Type: research
It would dedicate $1.5 billion of its total to research and therapy for Alzheimer ’s Disease, Parkinson’s Disease, stroke, epilepsy and other brain and central nervous system conditions.
Source: bizjournals.com Health Care:Pharmaceuticals headlines - Category: Pharmaceuticals Authors: Source Type: news
Publication date: Available online 8 October 2020Source: Hematology/Oncology and Stem Cell TherapyAuthor(s): Ram Vasudevan Nampoothiri, Shiyi Chen, Ivan Pasic, Zeyad Al-Shaibani, Wilson Lam, Fotios V. Michelis, Dennis Dong Hwan Kim, Auro Viswabandya, Armin Gerbitz, Jeffrey Howard Lipton, Rajat Kumar, Jonas Mattsson, Arjun Datt Law
Source: Hematology Oncology and Stem Cell Therapy - Category: Cancer & Oncology Source Type: research
Publication date: Available online 8 October 2020Source: Hematology/Oncology and Stem Cell TherapyAuthor(s): M.M. Sulaiman, A.I. Ladu, A.M. Abba, A.A. Bukar
Source: Hematology Oncology and Stem Cell Therapy - Category: Cancer & Oncology Source Type: research
Publication date: Available online 8 October 2020Source: Hematology/Oncology and Stem Cell TherapyAuthor(s): Muhammad Bilal Abid, Saurabh Chhabra, Blake Buchan, Mary Beth Graham, Sameem Abedin, Bicky Thapa, Anita D'Souza, Ben George, Mehdi Hamadani
Source: Hematology Oncology and Stem Cell Therapy - Category: Cancer & Oncology Source Type: research
ConclusionSOS is relatively rare; therefore, a holistic approach to the patients ’ needs considering nursing role as essential may result in better care outcomes.
Source: Supportive Care in Cancer - Category: Cancer & Oncology Source Type: research
It would dedicate $1.5 billion of its total to research and therapy for Alzheimer ’s Disease, Parkinson’s Disease, stroke, epilepsy and other brain and central nervous system conditions.
Source: bizjournals.com Health Care:Physician Practices headlines - Category: American Health Authors: Source Type: news
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