Association of proteome and metabolome signatures with severity in patients with community-acquired pneumonia.

Association of proteome and metabolome signatures with severity in patients with community-acquired pneumonia. J Proteomics. 2019 Dec 30;:103627 Authors: Salazar MG, Neugebauer S, Kacprowski T, Michalik S, Ahnert P, Creutz P, Rosolowski M, PROGRESS Study Group, Löffler M, Bauer M, Suttorp N, Kiehntopf M, Völker U Abstract A combined OMICS screening approach of human plasma and serum was used to characterize protein and metabolome signatures displaying association to severity of Community-acquired pneumonia (CAP). 240 serum and BD P100 EDTA plasma samples from patients diagnosed with CAP, collected during the day of enrolment to the hospital, were analyzed by a metabolomic and proteomic approach, respectively. Disease severity of CAP patients was stratified using the Sequential Organ Failure Assessment (SOFA) score. Quantitative proteome and metabolome data, derived by LC-MS/MS, were associated to SOFA and specific parameters of SOFA using linear regression models adjusted for age, BMI, sex, smoking and technical variables. Both proteome and metabolome profiling revealed remarkable strong changes in plasma and serum composition in relation to severity of CAP. Proteins and metabolites displaying SOFA associated levels are involved in immune response, particularly in processes of lipid metabolism. Proteins, which show an association to SOFA score, are involved in acute phase response, coagulation, complement activation and inflammatio...
Source: Journal of Proteomics - Category: Biochemistry Authors: Tags: J Proteomics Source Type: research