hnRNPA2B1 inhibits the exosomal export of miR-503 in endothelial cells.

hnRNPA2B1 inhibits the exosomal export of miR-503 in endothelial cells.
Cell Mol Life Sci. 2020 Jan 02;:
Authors: Pérez-Boza J, Boeckx A, Lion M, Dequiedt F, Struman I
Abstract
The chemotherapeutic drug epirubicin increases the exosomal export of miR-503 in endothelial cells. To understand the mechanisms behind this process, we transfected endothelial cells with miR-503 carrying a biotin tag. Then, we pulled-down the proteins interacting with miR-503 and studied their role in microRNA exosomal export. A total of four different binding partners were identified by mass spectrometry and validated by western blotting and negative controls, among them ANXA2 and hnRNPA2B1. Using knock-down systems combined with pull-down analysis, we determined that epirubicin mediates the export of miR-503 by disrupting the interaction between hnRNPA2B1 and miR-503. Then, both ANXA2 and miR-503 are sorted into exosomes while hnRNPA2B1 is relocated into the nucleus. The combination of these processes culminates in the increased export of miR-503. These results suggest, for the first time, that RNA-binding proteins can negatively regulate the exosomal sorting of microRNAs.
PMID: 31894362 [PubMed - as supplied by publisher]

Source: Cellular and Molecular Life Sciences : CMLS - January 1, 2020 Category: Cytology Authors: Pérez-Boza J, Boeckx A, Lion M, Dequiedt F, Struman I Tags: Cell Mol Life Sci Source Type: research