The challenge of classifying metastatic cell properties by molecular profiling exemplified with cutaneous melanoma cells and their cerebral metastasis from patient derived mouse xenografts.

The challenge of classifying metastatic cell properties by molecular profiling exemplified with cutaneous melanoma cells and their cerebral metastasis from patient derived mouse xenografts. Mol Cell Proteomics. 2019 Dec 31;: Authors: Neuditschko B, Janker L, Niederstaetter L, Brunmair J, Krivanek K, Izraely S, Sagi-Assif O, Meshel T, Keppler B, Del Favero G, Witz IP, Gerner C Abstract The prediction of metastatic properties from molecular analyses still poses a major challenge. Here we aimed at the classification of metastasis-related cell properties by proteome profiling making use of cutaneous and brain-metastasizing variants from single melanomas sharing the same genetic ancestry. Previous experiments demonstrated that cultured cells derived from these xenografted variants maintain a stable phenotype associated with a differential metastatic behavior: The brain metastasizing variants produce more spontaneous micro-metastases than the corresponding cutaneous variants. Four corresponding pairs of cutaneous and metastatic cells were obtained from four individual patients, resulting in eight cell-lines presently investigated. Label free proteome profiling revealed significant differences between corresponding pairs of cutaneous and cerebellar metastases from the same patient. Indeed, each brain metastasizing variant expressed a number of apparently metastasis-associated proteomic alterations as compared to the corresponding cutaneous ...
Source: Molecular and Cellular Proteomics : MCP - Category: Molecular Biology Authors: Tags: Mol Cell Proteomics Source Type: research