Immunologic detection of Lyme disease and the related borrelioses

Publication date: Available online 31 December 2019Source: Methods in MicrobiologyAuthor(s): Charles S. PaviaAbstractLyme disease and the relapsing fever borrelioses are caused by spirochetal bacteria belonging to the genus Borrelia. They have a partially restricted worldwide distribution depending upon their species. They are transmitted to people following exposure to the following arthropod vectors: ticks (for Lyme disease and endemic relapsing fever) and lice (for epidemic relapsing fever). Soon after a cutaneous infection becomes established, these spirochetes disseminate via the haematogenous route and are capable of causing multi-system disturbances that can vary in their degree of morbidity. Unlike most bacteria, the Borrelia are somewhat difficult to culture, which often makes isolating them from a patient specimen for diagnostic purposes a generally low-yield procedure. They stain poorly or cannot be stained at all by the gram stain technique, but they can be readily visualized using Wright's or Giemsa stain. Various laboratory-based detection systems are available to the healthcare community for diagnostic purposes, and many of them are immunologically based, relying heavily on serologic techniques. These tests are well described in this chapter, along with an in-depth analysis of their advantages and potential pitfalls. For Lyme disease, serologic techniques have proven to be indispensable for confirming or to rule out the diagnosis especially in troublesome cases...
Source: Methods in Microbiology - Category: Microbiology Source Type: research

Related Links:

Authors: Arab JP, Dirchwolf M, Álvares-da-Silva MR, Barrera F, Benítez C, Castellanos-Fernandez M, Castro-Narro G, Chavez-Tapia N, Chiodi D, Cotrim H, Cusi K, de Oliveira CPMS, Díaz J, Fassio E, Gerona S, Girala M, Hernandez N, Marciano S, Masson W, Mendez-Sanchez N, Leite N, Lozano A, Padilla M, Panduro A, Paraná R, Parise E, Perez M, Poniachik J, Restrepo JC, Ruf A, Silva M, Tagle M, Tapias M, Torres K, Vilar-Gomez E, Costa Gil JE, Gadano A, Arrese M Abstract Non-alcoholic fatty liver disease (NAFLD) currently represents an epidemic worldwide. NAFLD is the most frequently diagnosed chr...
Source: Annals of Hepatology - Category: Gastroenterology Tags: Ann Hepatol Source Type: research
This study aimed to evaluate in vitro and in vivo the wound healing effects of babassu oil. In vitro, babassu oil increased the migration of L929 fibroblasts, inhibited the production of nitric oxide by LPS-stimulated peritoneal macrophages, and increased the levels of INF-γ and IL-6 cytokines production. In vivo, babassu oil accelerated the healing process in a full-thickness splinted wound model, by an increase in the fibroblasts number, blood vessels, and collagen deposition in the wounds. The babassu oil also increased the recruitment of inflammatory cells into the wound site and showed an anti-inflammatory effec...
Source: Evidence-based Complementary and Alternative Medicine - Category: Complementary Medicine Tags: Evid Based Complement Alternat Med Source Type: research
After being epidemic in China, Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-COV-2) infection has rapidly spread in many countries as a global pandemic, with the number of affected cases dramatically increasing worldwide on a daily basis. Although the median age of hospitalized patients with confirmed infection is usually more advanced 1, with older age reported to be associated to higher mortality rate 2, physiological adaptations occurring during pregnancy have been claimed to be potentially responsible for a more severe respiratory disease, thus leading to higher rates of maternal and fetal complications 3,4.
Source: European Journal of Obstetrics, Gynecology, and Reproductive Biology - Category: OBGYN Authors: Tags: Correspondence Source Type: research
Authors: Stanhope J, Weinstein P PMID: 33029790 [PubMed - as supplied by publisher]
Source: Medical Journal of Australia - Category: General Medicine Tags: Med J Aust Source Type: research
On Oct. 1, New York state released an app that can notify you if you’ve come into contact with a person who has tested positive for COVID-19. Called “COVID Alert NY,” the app is one of 10 currently active in states around the U.S. that are based on Google and Apple’s decentralized contact tracing system, which was developed to maintain privacy while also giving health authorities a potentially powerful new tool to clamp down on outbreaks of the virus. “Everybody’s wondering, ‘I was next to this person, I was next to that person,’ but this can actually give you some data,&rdqu...
Source: TIME: Health - Category: Consumer Health News Authors: Tags: Uncategorized COVID-19 feature Londontime Source Type: news
Open letter calling for new Covid-19 strategy also signed by ‘Prof Cominic Dummings’Coronavirus – latest updatesSee all our coronavirus coverageAn open letter that made headlines calling for a herd immunity approach to Covid-19 lists a number of apparently fake names among its expert signatories, including “Dr Johnny Bananas” and “Professor Cominic Dummings”.TheGreat Barrington declaration, which was said to have been signed by more than 15,000 scientists and medical practitioners around the world, was found by Sky News to contain numerous false names, as well as those of several h...
Source: Guardian Unlimited Science - Category: Science Authors: Tags: Coronavirus outbreak Health UK news Science Epidemics Politics Health policy Infectious diseases Society Medical research World news US news Source Type: news
Conditions:   Virus-HIV;   Covid19 Intervention:   Other: questionnaire Sponsor:   Tourcoing Hospital Not yet recruiting
Source: ClinicalTrials.gov - Category: Research Source Type: clinical trials
Publication date: January 2021Source: Technological Forecasting and Social Change, Volume 162Author(s): Zhu Tiejun
Source: Technological Forecasting and Social Change - Category: Science Source Type: research
da HR Abstract The Northeast of Brazil has experienced a triple epidemic, with the simultaneous circulation of dengue virus (DENV), chikungunya virus (CHIKV) and Zika virus (ZIKV), which may have contributed to the observed increase across this region of atypical forms of disease and deaths. In view of this fact, non-congenital neurological disorders related to arboviruses were compared with other etiologies, mortality and survival rates of patients admitted to referral neurology hospitals in Pernambuco State, Northeast Brazil, from 2015 to 2018. Blood and cerebrospinal fluid samples were collected and tested usin...
Source: Revista do Instituto de Medicina Tropical de Sao Paulo - Category: Tropical Medicine Authors: Tags: Rev Inst Med Trop Sao Paulo Source Type: research
Abstract Asymptomatic VL is a concern, considering the risk of transmission in highly endemic areas due to human-to-human transmission. The aim of this study was to report the sero-epidemiological prevalence in Bihar, India, a highly endemic area of VL, using the leishmanin skin test (LST) and the direct agglutination test (DAT). This was a cross-sectional study performed in Muzaffarpur, Bihar, India. Relatives of patients with VL were tested by LST and DAT. Other epidemiological data were evaluated and correlated with tests results. Forty individuals (either previous or current patients), and 109 household contac...
Source: Revista do Instituto de Medicina Tropical de Sao Paulo - Category: Tropical Medicine Authors: Tags: Rev Inst Med Trop Sao Paulo Source Type: research
More News: Allergy & Immunology | Borrelia | Endemics | Epidemics | Epidemiology | Laboratory Medicine | Lice | Lyme Disease | Microbiology