Ultrasensitive impedimetric imunosensor for influenza A detection

Publication date: Available online 28 December 2019Source: Journal of Electroanalytical ChemistryAuthor(s): Aneta Anna Dunajová, Miroslav Gál, Kornélia Tomčíková, Romana Sokolová, Viliam Kolivoška, Eva Vanečková, Filip Kielar, František Kostolanský, Eva Varečková, Monika NaumowiczAbstractAcute respiratory infections epidemics are yearly caused by influenza A viruses due to their high variability. The course of disease can be sometimes very severe, especially in high risk groups of patients (suffering by chronical disease, immunosupression, or patients of age over 65). The World Health Organization (WHO) estimates that annually 250–500 thousand human deaths are caused globally by the influenza viral infections. To prevent the epidemic/pandemic spread of influenza infections, careful monitoring of epidemic viruses circulating in human population is required with the aim to prepare the effective influenza vaccine. This requires an early and very sensitive diagnostics. Therefore new, rapid diagnostic methods of high sensitivity and clinical specificity are continually developed.The goal of this work was to create an ultra-sensitive and highly selective impedimetric imunobiosensor for the detection of influenza A viruses based on the interaction with monoclonal antibodies, using disposable, easy to use screen printed carbon electrodes. Electrochemical impedance spectroscopy was used to characterize the sensors and describe their basic properties. Limit of detection...
Source: Journal of Electroanalytical Chemistry - Category: Chemistry Source Type: research