Paper-based nucleic acid testing system for simple and early diagnosis of mosquito-borne RNA viruses from human serum

Publication date: Available online 28 December 2019Source: Biosensors and BioelectronicsAuthor(s): Bhagwan S. Batule, Youngung Seok, Min-Gon KimAbstractThe recent outbreaks of mosquito-borne diseases (e.g., zika, dengue, and chikungunya) increased public health burden in developing countries. To control the spread of these infectious diseases, a simple, economic, reliable, sensitive, and selective diagnostic platform is required. Considering demand for affordable and accessible methods, we have demonstrated a two-step strategy for extraction and detection of viral RNAs of infectious diseases within 1 h. Ready-to-use devices for viral RNA extraction and detection were successfully fabricated using paper as a substrate. Viral RNA (e.g., zika, dengue, and chikungunya) was captured and eluted using a handheld RNA extraction paper-strip device, and another paper-chip device was used for reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay with a detection limit of a single copy and 10 copies of viral RNA in phosphate buffer solution (PBS) and serum, respectively. With these proposed devices, we have detected viral RNAs of zika and dengue in clinical human serum samples. The proposed paper-based extraction and detection platforms could be employed for detection of infectious viral diseases from complex clinical samples in resource-limited settings.Graphical abstract
Source: Biosensors and Bioelectronics - Category: Biotechnology Source Type: research

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Conclusion: These results suggest that if no vector control measures are considered to be in place, the risk of new arboviral outbreaks in Cabo Verde is high. The vector control strategy adopted must include measures of public health directed to domestic water storage and management.
Source: International Journal of Environmental Research and Public Health - Category: Environmental Health Authors: Tags: Article Source Type: research
Abstract The recent outbreaks of mosquito-borne diseases (e.g., zika, dengue, and chikungunya) increased public health burden in developing countries. To control the spread of these infectious diseases, a simple, economic, reliable, sensitive, and selective diagnostic platform is required. Considering demand for affordable and accessible methods, we have demonstrated a two-step strategy for extraction and detection of viral RNAs of infectious diseases within 1 h. Ready-to-use devices for viral RNA extraction and detection were successfully fabricated using paper as a substrate. Viral RNA (e.g., zika, dengue, ...
Source: Biosensors and Bioelectronics - Category: Biotechnology Authors: Tags: Biosens Bioelectron Source Type: research
CONCLUSIONS: The results indicate climate zone to be an important determinant of the basic reproduction number, R0, for dengue, Zika virus, and chikungunya. The role of other factors as determinants of R0, such as methods, environmental and social conditions, and disease control, should be further investigated. The results suggest that R0 may increase in temperate regions in response to global warming, and highlight the increasing need for strengthening preparedness and control activities. PMID: 31927301 [PubMed - as supplied by publisher]
Source: Environmental Research - Category: Environmental Health Authors: Tags: Environ Res Source Type: research
After the outbreak in French Polynesia, Zika virus (ZIKV) explosive pandemic occurred in 2015, reports that ZIKV was linked to other diseases, especially neurological complications such as Guillain-Barré Syndrome (GBS), also burst in almost every continent, thereby turning into an international concern. In South America, the highest incidence occurred in Venezuela with approximately 680 cases of GBS after ZIKV infection. Currently, the main roots of this association remain unknown. In this regard, it is thought that the immune system plays a central role in such association through the host protection or even improv...
Source: Current Immunology Reviews - Category: Allergy & Immunology Source Type: research
CONCLUSION: The last past arboviruses outbreaks did not have a significant impact on blood supply in FP. The measures introduced to prevent arbovirus transmission by transfusion were able to maintain infectious safety for all blood products without impairing self-sufficiency. PMID: 31889619 [PubMed - as supplied by publisher]
Source: Transfusion Clinique et Biologique - Category: Hematology Authors: Tags: Transfus Clin Biol Source Type: research
Canadian Journal of Microbiology, e-First Articles. Zika virus (ZIKV) is a mosquito-borne virus that was first isolated from Zika forest, Uganda, in 1947. Since its inception, major and minor outbreaks have been documented from several parts of world. Aedes spp. mosquitoes are the primary vectors of ZIKV, but the virus can also be transmitted through sexual practices, materno-fetal transmission, and blood transfusion. The clinical presentations of symptomatic ZIKV infections are similar to dengue and chikungunya, including fever, headache, arthralgia, retro-orbital pain, conjunctivitis, and rash. ZIKV often causes mild ill...
Source: Canadian Journal of Microbiology - Category: Microbiology Authors: Source Type: research
This report shows the need for an efficient surveillance system to control the spread of this virus in the population and suggests the use of saliva and urine samples as an alternative for the detection of OROV when serum samples are not available. PMID: 31787741 [PubMed - as supplied by publisher]
Source: Japanese Journal of Infectious Diseases - Category: Infectious Diseases Authors: Tags: Jpn J Infect Dis Source Type: research
Authors: Maniero VC, Rangel PSC, Coelho LMC, Silva CSB, Aguiar RS, Lamas CC, Cardozo SV Abstract A progressive increase in the circulation of arboviruses in tropical countries has been observed, accounting for 700,000 yearly deaths in the world. The main objective of this article was to identify the presence of Zika (ZIKV), dengue (DENV), and Chikungunya (CHIKV) viruses in immature stages of Aedes aegypti and Ae. albopictus. Household collections of immature phases of the vectors were carried out in the years 2015 and 2016. A total of 2902 dwellings were visited and the rate of infestation with larvae and pupae of ...
Source: Brazilian Journal of Medical and Biological Research - Category: Research Tags: Braz J Med Biol Res Source Type: research
Conclusions: Aedes albopictus is responsible for the current outbreaks. The spread of Aedes albopictus and Aedes japonicus increases the risk of the autochthonous transmission of these viruses. Although native species could contribute to their transmission, more studies are still needed to assess that risk.
Source: Viruses - Category: Virology Authors: Tags: Review Source Type: research
While efforts to control malaria with available tools have stagnated, and arbovirus outbreaks persist around the globe, the advent of clustered regularly interspaced short palindromic repeat (CRISPR)-based gene editing has provided exciting new opportunities for genetics-based strategies to control these diseases. In one such strategy, called “population replacement”, mosquitoes, and other disease vectors are engineered with effector genes that render them unable to transmit pathogens. These effector genes can be linked to “gene drive” systems that can bias inheritance in their favor, providing nove...
Source: Frontiers in Genetics - Category: Genetics & Stem Cells Source Type: research
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