Assessment of variation in B-cell receptor heavy chain repertoire in patients with end-stage renal disease by high-throughput sequencing.
Assessment of variation in B-cell receptor heavy chain repertoire in patients with end-stage renal disease by high-throughput sequencing. Ren Fail. 2019 Nov;41(1):1-13 Authors: Wang L, Dai Y, Liu S, Lai L, Yan Q, Chen H, Zhang J, Sui W Abstract Background/Aims: End-stage renal disease (ESRD), characterized by progressive loss of rental function during the disease course, has been reported to be correlated with immune dysregulation. To date, a majority of previous studies on immune response to ESRD have been focused on the T-cell response. This prospective study was to assess the B-cell receptor (BCR) heavy chain repertoire in ESRD patients.Materials and methods: A total of 10 ESRD patients and six healthy controls were prospectively enrolled in this study. BCR immunoglobulin heavy chain (IGH) repertoire in the peripheral blood from ESRD patients and healthy individuals were analyzed by means of next generation sequencing (NGS) in combination with multiplex PCR, Illumina sequencing, and the international ImMunoGeneTics database (IMGT).Results: Abnormal BCR complementary-determining region 3 (CDR3) sequences were identified in relation to ESRD. We also found that the degree of the B-cell clonal expansion in the ESRD group was significantly greater than that in the control group (p
Ziting Wang, Pradeep Durai, Ho Yee TiongIndian Journal of Urology 2020 36(2):89-94There is an increasing gulf between demand and supply for kidneys in end-stage renal failure patients worldwide, especially Asia. Renal transplantation is often the treatment of choice for long-suffering patients who have to undergo dialysis on a regular basis. The utilization of expanded criteria donors (ECDs) to address the donor pool shortage has been proven to be a legitimate solution. Metzger first described the classification of standard criteria donor and ECD in 2002. Since then, the criterion has undergone various modifications, with ...
We present a 50-year-old male who underwent PCNL for renal pelvic calculus. Since the day of the surgery, he had low-grade fever (100°F) which worsened (102°F) from the 2nd postoperative day. His preoperative urine culture was sterile. His platelet counts started dropping and NS1 antigen for dengue was positive. He also developed anemia (hemoglobin: 7g%) and platelet counts dropped to 17,000/cmm. He developed anuria on the 7th postoperative day, with serum creatinine rising to 7 mg%. He required two sessions of hemodialysis and urine output improved. There is a need for high index of suspicion for dengue,...
Renovascular hypertension (RVH) associated with renal artery and abdominal aortic narrowings is the third most common cause of pediatric hypertension. Untreated children may experience major cardiopulmonary complications, stroke, renal failure and death. The impetus of this study was to describe the increasingly complex surgical practice for such patients with an emphasis on anatomic phenotype and contemporary outcomes following surgical management, as a means of identifying those factors responsible for persistent or recurrent hypertension necessitating reoperation.
Renal failure is a serious complication associated with multiple myeloma that can occur in up to 50% of cases at diagnosis.1 Although the introduction of novel antimyeloma agents has improved outcomes, severe renal impairment is still associated with an early risk of death in patients with multiple myeloma.2,3 Renal injury occurs in multiple myeloma secondary to the direct toxic effect of monoclonal light chains precipitating in the renal tubules.4 Other potential contributors to myeloma-related renal impairment include hypercalcemia, dehydration, and use of nephrotoxic agents such as analgesics, antibiotics, and contrast dye.
J Reconstr Microsurg DOI: 10.1055/s-0040-1709477 Background Overall success rate after diabetic foot reconstruction using free flap has been acceptable, but certain parts of the foot like the heel remains more challenging. Patients and Methods This retrospective study reviewed 37 patients reconstructed with free flaps for diabetic foot ulcer on the heel region from 2008 to 2017. Flap outcome in addition to various risk factors were analyzed; arterial status on heel, the American Society of Anesthesiologists (ASA) physical status, smoking, hypertension, hypercholesterolemia, chronic renal failure patient, he...
Myocardial calcifications are often discovered incidentally and result from dystrophic or metastatic deposition of calcium1. Dystrophic calcification represents the sequalae of local tissue damage with necrosis, most commonly from myocardial ischemia. Unlike the prior, metastatic calcification is the result of abnormal calcium homeostasis; including renal failure, hyperparathyroidism, and vitamin D deficiency. Myocardial calcifications are not entirely a benign finding, since they can lead to heart failure, arrhythmias and sudden cardiac death2.
Authors: Zhao WB, Zhu L, Rahman T Abstract Objective: Few studies have investigated the association of apolipoprotein B (ApoB) with the progression of diabetic kidney disease (DKD) and the risk of renal replacement therapy (RRT).Method: In this retrospective cohort study, a group of 258 DKD patients with stage 3-5chronic kidney disease(CKD)were divided into low ApoB (
CONCLUSIONS: The current research progress in classification, genetic heterogeneity, diagnosis, and treatment of Dent disease reviewed in this paper enables doctors and researchers to better understand Dent disease and provides a basis for improved prevention and treatment. PMID: 32248351 [PubMed - as supplied by publisher]
In conclusion, this mutant may be an effective vaccine candidate against scorpion envenomation by H. lepturus in future clinical studies.
Ignoring a problem won ’t make it go away. I say this all the time, with love and compassion, to my patients who are having trouble accepting a diagnosis. Ignoring your Type 2 Diabetes, for example, won’t make it any less real. It will only land you in renal failure and on dialysis, or with a […]Find jobs at Careers by KevinMD.com. Search thousands of physician, PA, NP, and CRNA jobs now. Learn more.