Formulations of Pseudomonas fluorescens and Burkholderia pyrrocinia control rice blast of upland rice cultivated under no-tillage system

The objective of this work was to evaluate the potential of four liquid formulations containing Pseudomonas fluorescens (BRM 32111) and Burkholderia pyrrocina (BRM 32113) to suppress leaf blast, under field conditions. The trials were conducted at Embrapa Rice and Beans, between December 2018 and April 2019. The experiments were performed in a randomized block containing 7 treatments, 4 replicates. The treatments consisted of microbiolized seeds and sprays at 14 and 21 days after planting with: Formulation 11 + BRM 32,113 (T1); Formulation 32 + BRM 32,113 (T2); Formulation 11 + BRM 32,111 (T3): Formulation 20 + BRM 32,111 (T4); BRM 32,111 (T5); BRM 32,113 (T6) and Absolute control (water – T7). Severity of leaf blast and panicle, gas exchange and grain yield were evaluated. Plants treated with P. fluorescens (T3, T4 and T5) or B. pyrrocinia (T1, T2 and T6) presented 49%, 39%, 60%, 15% increase in the assimilation rate of CO2 (A), transpiration (E), stomatal conductance (gs) and internal CO2 concentration (Ci), respectively. All treatments were efficient in suppressing leaf and panicle blast and in promoting biomass increase in 55%. The yields of T1, T4, T5 and T6 treatments increased 481, 424, 688 and 427 kg ha−1, respectively. Our results indicate that the formulated rhizobacteria have the potential to be included in the integrated management for blast control in rice fields.Graphical abstract
Source: Biological Control - Category: Biology Source Type: research

Related Links:

Burkholderia pseudomallei, the causative agent of melioidosis, can survive and replicate in macrophages. Little is known about B. pseudomallei genes that are induced during macrophage infection. We constructed a B. pseudomallei K96243 promoter trap library with genomic DNA fragments fused to the 5′ end of a plasmid-borne gene encoding enhanced green fluorescent protein (eGFP). Microarray analysis showed that the library spanned 88% of the B. pseudomallei genome. The recombinant plasmids were introduced into Burkholderia thailandensis E264, and promoter fusions active during in vitro culture were removed. J774A.1 muri...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
Leitão RNA-binding proteins (RBPs) are important regulators of cellular functions, playing critical roles on the survival of bacteria and in the case of pathogens, on their interaction with the host. RBPs are involved in transcriptional, post-transcriptional, and translational processes. However, except for model organisms like Escherichia coli, there is little information about the identification or characterization of RBPs in other bacteria, namely in members of the Burkholderia cepacia complex (Bcc). Bcc is a group of bacterial species associated with a poor clinical prognosis in cystic fibrosis patients. The...
Source: Genes - Category: Genetics & Stem Cells Authors: Tags: Article Source Type: research
Abstract Skatole is the key malodorous compound in livestock and poultry waste and wastewater with a low odor threshold. It not only causes serious nuisance to residents and workers, but also poses threat to the environment and human health due to its biotoxicity and recalcitrant nature. Biological treatment is an eco-friendly and cost-effective approach for skatole removal, while the bacterial resources are scarce. Herein, the Burkholderia strain was reported to efficiently degrade skatole for the first time. Results showed that strain IDO3 maintained high skatole-degrading performance under the conditions of pH ...
Source: Environmental Research - Category: Environmental Health Authors: Tags: Environ Res Source Type: research
aacute;nchez E, Ortiz-Hernández ML Abstract Pesticides are xenobiotic molecules necessary to control pests in agriculture, home, and industry. However, water and soil can become contaminated as a consequence of their extensive use. Therefore, because of its eco-friendly characteristics and efficiency, bioremediation of contaminated sites is a powerful tool with advantages over other kinds of treatments. For an efficient pesticides bioremediation, it is necessary to take into account different aspects related to the microbial metabolism and physiology. In this respect, OMICs studies such as genomics, transcr...
Source: Current Microbiology - Category: Microbiology Authors: Tags: Curr Microbiol Source Type: research
Melioidosis is an infectious disease with a high mortality rate responsible for community-acquired sepsis in Southeast Asia and Northern Australia. The causative agent of this disease is Burkholderia pseudomallei, a Gram-negative bacterium that resides in soil and contaminated natural water. After entering into host cells, the bacteria escape into the cytoplasm, which has numerous cytosolic sensors, including the noncanonical inflammatory caspases. Although the noncanonical inflammasome (caspase-11) has been investigated in a murine model of B. pseudomallei infection, its role in humans, particularly in lung epithelial cel...
Source: Infection and Immunity - Category: Infectious Diseases Authors: Tags: Cellular Microbiology: Pathogen-Host Cell Molecular Interactions Source Type: research
P. Rongkard et al.
Source: Emerging Infectious Diseases Journal - Category: Infectious Diseases Source Type: research
Publication date: Available online 8 February 2020Source: Veterinary MicrobiologyAuthor(s): Priyangi A Alwis, Puthayalai Treerat, Lan Gong, Deanna Deveson Lucas, Elizabeth M Allwood, Mark Prescott, Rodney J Devenish, Ben Adler, John D BoyceAbstractTwo-component signal transduction systems (TCSTS) are abundant among prokaryotes and regulate important functions, including drug resistance and virulence. The Gram-negative bacterium Burkholderia pseudomallei, which causes the severe infectious disease melioidosis, encodes 136 putative TCSTS components. In silico analyses of these TCSTS indicated that the predicted BbeR-BbeS sys...
Source: Veterinary Microbiology - Category: Veterinary Research Source Type: research
Abstract A spider-transmitted fungus ( Rhizopus microsporus ) that was isolated from necrotic human tissue was found to harbor endofungal bacteria ( Burkholderia sp.). Metabolic profiling of the symbionts revealed a complex of cytotoxic agents (necroximes). Their structures were fully characterized as oxime-substituted benzolactone enamides equipped with a peptidic side chain. Necroximes are potent cytotoxic agents that are also formed in symbiosis with the fungal host and could have contributed to the necrosis. Genome sequencing and in silico analyses revealed a novel modular PKS/NRPS assembly line equipped with ...
Source: Angewandte Chemie - Category: Chemistry Authors: Tags: Angew Chem Int Ed Engl Source Type: research
In this study, the therapeutic potential of combinations of an antibiotic and a broad-spectrum antimicrobial peptide (AMP) was evaluated against five bacterial biothreats, the etiologic agents of glanders (Burkholderia mallei), melioidosis (Burkholderia pseudomallei), plague (Yersinia pestis), tularemia (Francisella tularensis), and anthrax (Bacillus anthracis). The therapeutics included licensed early generation antibiotics which are now rarely used. Three antibiotics and one 24- amino acid AMP were selected based on MIC assay data. Combinations of the AMP and tigecycline, minocycline, or novobiocin were screened for syne...
Source: Microbial Pathogenesis - Category: Infectious Diseases Source Type: research
Abstract Biomass production and metal accumulation in plant tissue (bioconcentration) are two critical factors limiting the phytoextraction rate. Metal translocation to aboveground organs should be accounted for as the third most important factor, as harvesting of the plant roots is usually economically disadvantageous. These three parameters could be potentially increased with the use of companion planting, a well-known agricultural technique, and inoculation with plant growth-promoting bacteria (PGPB). The aim of the study was to determine whether intercropping and inoculation with endophytic PGPB (Burkholderia ...
Source: Environmental Science and Pollution Research International - Category: Environmental Health Authors: Tags: Environ Sci Pollut Res Int Source Type: research
More News: Biology | Burkholderia