Phenothiazinium Dyes Are Active against Trypanosoma cruzi In Vitro.

Phenothiazinium Dyes Are Active against Trypanosoma cruzi In Vitro. Biomed Res Int. 2019;2019:8301569 Authors: Bulhões Portapilla G, Pereira LM, Bronzon da Costa CM, Voltarelli Providello M, Sampaio Oliveira PA, Goulart A, Ferreira Anchieta N, Wainwright M, Leite Braga GÚ, de Albuquerque S Abstract Chagas disease is a tropical illness caused by the protozoan Trypanosoma cruzi. The disease affects populations of the Americas and has been spread to other continents due to the migration process. The disease is partially controlled by two drugs, Benznidazole and Nifurtimox. These molecules are active in the acute phase of the infection but are usually ineffective during the symptomatic chronic phase. Several research groups have developed novel candidates to control Chagas disease; however, no novel commercial formulation is available. In this article, we described the anti-T. cruzi effects of phenothiazinium dyes in amastigote and trypomastigote forms of the parasite. Methylene Blue, New Methylene Blue, Toluidine Blue O, and 1,9-Dimethyl Methylene Blue inhibited the parasite proliferation at nanomolar concentrations and also demonstrated low toxicity in host cells. Moreover, combinations of phenothiazinium dyes indicated a synergic pattern against amastigotes compared to the Benznidazole counterparts. Phenothiazinium dyes levels of reactive oxygen species (ROS) and decreased the mitochondrial potential in trypomastigotes, indicating the mechan...
Source: Biomed Res - Category: Research Authors: Tags: Biomed Res Int Source Type: research

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