Theileria annulata transformation altered cell surface molecules expression and endocytic function of monocyte-derived dendritic cells

Publication date: Available online 26 December 2019Source: Ticks and Tick-borne DiseasesAuthor(s): Junlong Liu, Muhammad Rashid, Jinming Wang, Aihong Liu, Guiquan Guan, YouQuan Li, Lan He, Hong Yin, Jianxun LuoAbstractTheileria annulata is a protozoan parasite transmitted by ticks to cattle. The most important processes of T. annulata are the infection and transformation of host monocytes, which promote cells division and generate a neoplastic phenotype. Dendritic cells play an important role in the development of adaptive immune responses against parasites and are traditionally classified into four types. One type of dendritic cell derived from afferent lymph was successfully transformed by T. annulata in vitro in previously report. However, whether the monocyte-derived dendritic cells could be transformed and how the endocytic function is affected by T. annulata infection were not yet known. Bovine dendritic cells (DCs) derived from blood CD14+ monocytes were cocultured with T. annulata sporozoites in vitro. On day 15 post infection, rounded and continuously proliferating cells were observed. The effect of this transformation on cell phenotype was studied using immunostaining and flow cytometry. After transformation, the cells maintained the expression of the DC-specific marker CD11c, but it was down-regulated as were the expression of CD11b, CD14 and CD86. In contrast, CD205, CD45 and MHC class Ⅱ molecules were upregulated in transformed cells. The levels of CD172a, CD21...
Source: Ticks and Tick borne Diseases - Category: Zoology Source Type: research