Molecular characterization of enterocin EF35 against human pathogens and its in-silico analysis against human cancer proteins TOP1 and PI3K

Publication date: Available online 27 December 2019Source: Biocatalysis and Agricultural BiotechnologyAuthor(s): Rajesh P. Shastry, R.R. Arunrenganathan, V. Ravishankar RaiAbstractEnterococcus faecalis is a common commensal of the gastrointestinal tract and produce different types of bacteriocins. Here, we describe En. faecalis strain from fresh water fish, which produce a bacteriocin significantly (p < 0.05) inhibited Staphylococcus aureus, Salmonella enterica ser. Typhi and Escherichia coli. Degenerate primers were used to amplify the bacteriocin encoding gene, showed that the presence of enterocin gene was homologous to the class II bacteriocins. Enterocin gene revealed that the presence of antibacterial conserved regions YGNGV motif at the N-terminal region. Docking studies were performed on the predicted enterocin EF35 and two human cancer protein TOP 1 and PI3K. The molecular simulation showed that the ligand strongly inhibits the enzymes TOP 1 and PI3K with the binding affinity of −13.2 and −11.1 kcal/mol respectively. In-vitro experiments suggest the potential application of enterocin EF35 in control of human pathogens and In-Silico analysis addressed the importance of identified peptide in inhibition of human topoisomerase 1 and phosphoinositide 3-kinase.
Source: Biocatalysis and Agricultural Biotechnology - Category: Biotechnology Source Type: research