GSE98979 Dissecting molecular mediators of TGFb- and HGF-induced cell mechanical enhancement in NSCLC

Contributors : Hauke Busch ; Melanie Boerries ; Ursula Klingm üller ; Evgeny Gladilin ; Kai Breuhahn ; Ruth Merkle ; Dmytro DvornikovSeries Type : Expression profiling by arrayOrganism : Homo sapiensImportance of growth factor (GF) signaling in cancer progression is widely acknowledged. Transforming growth factor beta (TGF β) and and hepa- tocyte growth factor (HGF) are known to play a key role in epithelial-to- mesenchymal transition (EMT) and metastatic cell transformation that are characterized by distinctive alterations in cell mechanical architecture and behavior towards a more robust and motile single cell phen otype. How- ever, molecular mechanisms mediating cancer type specific enhancement of cell mechanical properties in response to growth factors remain unlighted. Here, we combine high-throughput mechanical cell phenotyping, microarray analysis and gene-silencing to dissect molecular mediators of GF-in duces cell mechanical enhancement in non-small-cell lung adenocarcinoma (NSCLC). Our experimental results show that both growth factors elevate cell rigidity, size and migratory activity, however, effects of TGFβ stimulation on cell mechanical phenotype and differential gene expression turn out to be significantly stronger. Our microarray analysis reveals that TGFβ-treated NSCLC cells exhibit upregulation of migration-, adhesion-related and down- regulation of cytokinesis-related groups of genes. In addition to vimentin, a canonical marker of EMT, a large n...
Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Expression profiling by array Homo sapiens Source Type: research