Development and Analytical Validation of a DNA Dual-Strand Approach for the US Food and Drug Administration –Approved Next-Generation Sequencing–Based Praxis Extended RAS Panel for Metastatic Colorectal Cancer Samples

A next-generation sequencing method was developed that can distinguish single-stranded modifications from low-frequency somatic mutations present on both strands of DNA in formalin-fixed paraffin-embedded colorectal cancer samples. We applied this method for analytical validation of the Praxis Extended RAS Panel, a US Food and Drug Administration –approved companion diagnostic for panitumumab, on the Illumina MiSeqDx platform. With the use of the TruSeq amplicon workflow, both strands of DNA from the starting material were interrogated independently.

Source: Journal of Molecular Diagnostics - December 10, 2019 Category: Pathology Authors: Nitin Udar, Anita Iyer, Margaret Porter, Robert Haigis, Shannon Smith, Shivani Dhillon, Kristen Meier, Diane Ward, Jing Lu, Paul Wenz, Leonard Buchner, Tamsen Dunn, Aaron Wise, Amy Mueller, Karen Gutekunst Tags: Regular article Source Type: research