Detection of antibodies to Toxoplasma gondii in oral fluid from pigs.

Detection of antibodies to Toxoplasma gondii in oral fluid from pigs.
Int J Parasitol. 2019 Dec 19;:
Authors: Campero LM, Schott F, Gottstein B, Deplazes P, Sidler X, Basso W
Abstract
Toxoplasma gondii-infected pigs play a major role as a source of infection for humans and detection of high-risk herds is essential to implement control measures at the farm level. The aim of this study was to determine whether oral fluid (OF) could be used as a matrix to detect antibodies against T. gondii in infected pigs by immunoblot (IB). For this, OF from experimentally inoculated sows (n=8) (serial samples) and naturally exposed group-housed fatteners (n=42 groups, one sample/group) were analysed for IgG and IgA against T. gondii-SAG1 antigen by IB. Simultaneously, each animal was serologically tested for anti-T. gondii IgG by ELISA. Specific IgG was detected in the sera of all inoculated sows from 2-3 weeks post inoculation (pi) and in 3.4 to 92% of the pigs in 13 out of 42 groups. Experimentally inoculated sows showed positive OF-IB results for IgA (100%) and IgG (87.5%) at 1.5 weeks pi and continued yielding positive results for IgA (87.5-75%) and IgG (50%) until 4 weeks pi; however, from 8 weeks pi the frequency of detection of both isotypes was lower, despite constantly positive IgG values in serum-ELISA. Interestingly, consecutive daily samplings for 4 days at 13 and 30 weeks pi showed inconsistent results for some sows, showing that the an...

Source: International Journal for Parasitology - December 18, 2019 Category: Parasitology Authors: Campero LM, Schott F, Gottstein B, Deplazes P, Sidler X, Basso W Tags: Int J Parasitol Source Type: research