Digital Rolling Circle Amplification–Based Detection of Ebola and Other Tropical Viruses

Publication date: Available online 16 December 2019Source: The Journal of Molecular DiagnosticsAuthor(s): Sibel Ciftci, Felix Neumann, Samir Abdurahman, K. Sofia Appelberg, Ali Mirazimi, Mats Nilsson, Narayanan MadaboosiEmerging tropical viruses have caused serious outbreaks during the recent years, such as Ebola virus (EBOV) in 2014 and the most recent in 2018 to 2019 in Congo. Thus, immediate diagnostic attention is demanded at the point of care in resource-limited settings, because the performance and the operational parameters of conventional EBOV testing are limited. Especially, their sensitivity, specificity, and coverage of other tropical disease viruses make them unsuitable for diagnostic at the point of care. Here, a padlock probe (PLP)-based rolling circle amplification (RCA) method for the detection of EBOV is presented. For this, a set of PLPs, separately targeting the viral RNA and complementary RNA of all seven EBOV genes, was used in the RCA assay and validated on virus isolates from cell culture. The assay was then translated for testing clinical samples, and simultaneous detection of both EBOV RNA types was demonstrated. For increased sensitivity, the RCA products were enriched on a simple and pump-free microfluidic chip. Because PLPs and RCA are inherently multiplexable, we demonstrate the extension of the probe panel for the simultaneous detection of the tropical viruses Ebola, Zika, and Dengue. The demonstrated high specificity, sensitivity, and multiplexi...
Source: The Journal of Molecular Diagnostics - Category: Pathology Source Type: research

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In the last decades, a number of infectious viruses have emerged from wildlife or re-emerged, generating serious threats to the global health and to the economy worldwide. Ebola and Marburg hemorrhagic fevers, Lassa fever, Dengue fever, Yellow fever, West Nile fever, Zika, and Chikungunya vector-borne diseases, Swine flu, Severe acute respiratory syndrome (SARS), Middle East respiratory syndrome (MERS), and the recent Coronavirus disease 2019 (COVID-19) are examples of zoonoses that have spread throughout the globe with such a significant impact on public health that the scientific community has been called for a rapid int...
Source: Frontiers in Immunology - Category: Allergy & Immunology Source Type: research
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Source: MEDICC Review - Category: International Medicine & Public Health Tags: MEDICC Rev Source Type: research
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Source: The Health Care Blog - Category: Consumer Health News Authors: Tags: COVID-19 Health Policy Chadi Nabhan epidemic Pandemic Zika Source Type: blogs
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Source: Medgadget - Category: Medical Devices Authors: Tags: Diagnostics Exclusive Public Health Source Type: blogs
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Publication date: Available online 16 December 2019Source: The Journal of Molecular DiagnosticsAuthor(s): Sibel Ciftci, Felix Neumann, Samir Abdurahman, K. Sofia Appelberg, Ali Mirazimi, Mats Nilsson, Narayanan MadaboosiAbstractEmerging tropical viruses have caused serious outbreaks during the recent years, such as Ebola virus (EBOV) in 2014 and the most recent in 2018-19 in Congo. Thus, immediate diagnostic attention is demanded at the point-of-care in resource-limited settings, since the performance and the operational parameters of conventional EBOV testing are limited. Especially, their sensitivity, specificity and cov...
Source: The Journal of Molecular Diagnostics - Category: Pathology Source Type: research
A Danish and Taiwanese company has won Australia’s Good Design Award for a portable diagnostic device—small enough to fit in a camera case—that allows diagnosis of infectious diseases on the spot from a single drop of blood. “The Good Design Award ranked BluBox as the best solution for the rapid and accurate assessment of communicable diseases in heavily populated as well as resource-constrained, expertise-lacking areas,” explained Filippo Bosco, CEO and one of the three founders of BluSense Diagnostics (Copenhagen). The secret inside...
Source: MDDI - Category: Medical Devices Authors: Tags: IVD Source Type: news
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Source: JAMA Ophthalmology - Category: Opthalmology Source Type: research
In this study, we evaluated ATA as a potential antiviral drug against ZIKV replication. The antiviral activity of ATA against ZIKV replication in vitro showed median inhibitory concentrations (IC50) of 13.87 ± 1.09 μM and 33.33 ± 1.13 μM in Vero and A549 cells, respectively; without showing any cytotoxic effect in both cell lines (median cytotoxic concentration (CC50)> 1,000 μM). Moreover, ATA protected both cell types from ZIKV-induced cytopathic effect (CPE) and apoptosis in a time- and concentration-dependent manner. In addition, pre-treatment of Vero cells with ATA for up to 72 h also resulted...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
Adriano Queiroz1, Isabella Fernanda Dantas Pinto2, Maricélia Lima3, Marta Giovanetti1,4, Jaqueline Goes de Jesus1, Joilson Xavier1, Fernanda Khouri Barreto5, Gisele André Baptista Canuto6, Helineide Ramos do Amaral7, Ana Maria Bispo de Filippis4, Denise Lima Mascarenhas8, Melissa Barreto Falcão8, Normeide Pedreira Santos8, Vasco Ariston de Carvalho Azevedo9, Marcos Yukio Yoshinaga2*, Sayuri Miyamoto2 and Luiz Carlos Junior Alcantara1,4,10* 1Laboratório de Patologia Experimental, Instituto Gonçalo Moniz, Salvador, Brazil 2Departamento de Bioquímica, Instituto de Quími...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
More News: Congo Health | Dengue Fever | Ebola | Genetics | Outbreaks | Pathology | Zika Virus