Development of a simple and rapid immunochromatographic strip test for detecting duck plague virus antibodies based on gI protein.

Development of a simple and rapid immunochromatographic strip test for detecting duck plague virus antibodies based on gI protein.
J Virol Methods. 2019 Dec 18;:113803
Authors: Zhou X, Wang M, Cheng A, Yang Q, Ying W, Jia R, Liu M, Zhu D, Chen S, Zhang S, Zhao XX, Huang J, Mao S, Xumin O, Gao Q, Liu Y, Yanling Y, Zhang L, Tian B, Pan L, Rehman MU, Chen X
Abstract
A colloidal gold strip (CGS) for detecting antibodies to duck plague virus (DPV) was developed. Colloidal gold-labeled DPV gI protein and goat anti-rabbit IgG were dispensed on a conjugate pad as tracers. The recombinant DPV gI protein and rabbit IgG were used as capture reagents at the test line and control line, respectively. The detection limit of this assay was 1:256. Additionally, the CGS did not react with antisera from other common duck diseases, only reacting with anti-DPV serum and yielding a specific and strong red signal. 123 serum samples were tested by CGS and enzyme-linked immunosorbent assay (ELISA), and the results showed good agreement. The CGS test results can be observed in 15 min with the naked eye, should be suitable for clinical testing and large-scale detection.
PMID: 31863862 [PubMed - as supplied by publisher]

Source: Journal of Virological Methods - December 17, 2019 Category: Virology Authors: Zhou X, Wang M, Cheng A, Yang Q, Ying W, Jia R, Liu M, Zhu D, Chen S, Zhang S, Zhao XX, Huang J, Mao S, Xumin O, Gao Q, Liu Y, Yanling Y, Zhang L, Tian B, Pan L, Rehman MU, Chen X Tags: J Virol Methods Source Type: research