Pseudomonas aeruginosa mexT is an indicator of PAO1 strain integrity.

Pseudomonas aeruginosa mexT is an indicator of PAO1 strain integrity. J Med Microbiol. 2019 Dec 20;: Authors: LoVullo ED, Schweizer HP Abstract Laboratory research with Pseudomonas aeruginosa commonly involves the prototype strain PAO1. There is continued concern that PAO1 sublines maintained and propagated in the same laboratory or different laboratories exhibit genetic and phenotypic variability that may affect the reproducibility and validity of research. Whole-genome sequencing and other research identified the mexT locus as a mutational hotspot, but the explication of the diverse mutations present in the various sublines and consequences remained rather cursory. Here we present evidence that MexT sequence diversity is a predictor of PAO1 lineage integrity and define the protein's prototype sequence. PMID: 31859619 [PubMed - as supplied by publisher]
Source: Journal of Medical Microbiology - Category: Microbiology Authors: Tags: J Med Microbiol Source Type: research