MicroRNA-30d-5p promotes ovarian granulosa cell apoptosis by targeting Smad2.

MicroRNA-30d-5p promotes ovarian granulosa cell apoptosis by targeting Smad2. Exp Ther Med. 2020 Jan;19(1):53-60 Authors: Yu M, Liu J Abstract Polycystic ovarian syndrome (PCOS) is one of the leading causes of female infertility. MicroRNA-30d-5p (miR-30d-5p) has been reported to be significantly increased during follicle stimulating hormone (FSH)-mediated progesterone secretion of cultured granulosa cells. However, its role in the proliferation and apoptosis of ovarian granulosa cells is unclear. The present study aimed to investigate the role of miR-30d-5p in the proliferation and apoptosis of ovarian granulosa cells. Bioinformatic analysis and dual-luciferase reporter assay were used to predict and confirm the direct target of miR-30d-5p. The levels of miR-30d-5p were detected via reverse transcription-quantitative PCR (RT-qPCR), cell proliferation was detected via an MTT assay and cell apoptosis was measured via flow cytometry. The levels of phosphorylated (p)-Smad2, Smad2, p-Smad3 and Smad3 were detected by performing a western blot assay or RT-qPCR. In the present results, Smad2 was identified as the direct and functional target of miR-30d-5p. Compared with the control and control plasmid groups, the Smad2 plasmid significantly enhanced Smad2 mRNA levels in rat ovarian granulosa cells, enhanced rat ovarian granulosa cell viability and reduced cell apoptosis. In addition, the results demonstrated that overexpression of miR-30d-5p...
Source: Experimental and Therapeutic Medicine - Category: General Medicine Tags: Exp Ther Med Source Type: research