Quantitative determination of bilobetin in rat plasma by HPLC-MS/MS and its application to a pharmacokinetic study.

Quantitative determination of bilobetin in rat plasma by HPLC-MS/MS and its application to a pharmacokinetic study. Biomed Chromatogr. 2019 Dec 18;:e4784 Authors: Son H, Kang W Abstract Although bilobetin, a biflavone isolated from the leaves of Ginkgo biloba, represents a variety of pharmacological activities, to date, there have been no validated determination methods of bilobetin in biological samples. Thus, we developed a liquid chromatographic method using a tandem mass spectrometry for the determination of bilobetin in rat plasma. After protein precipitation with acetonitrile including diclofenac (internal standard), the analytes were chromatographed on a reversed-phased column with a mobile phase of purified water and acetonitrile (3:7, v/v, including 0.1% formic acid). The ion transitions of the precursor to the product ion were principally deprotonated ions [M-H]- at m/z 551.2→519.2 for bilobetin and 296.1→251.7 for the IS. The accuracy and precision of the assay were in accordance with FDA regulations for the validation of bioanalytical methods. This analytical method was successfully applied to monitor plasma concentrations of bilobetin over time following intravenous administration in rats. PMID: 31853982 [PubMed - as supplied by publisher]
Source: Biomedical Chromatography : BMC - Category: Biomedical Science Authors: Tags: Biomed Chromatogr Source Type: research