Assessment of differences between DNA content of cell-cultured and freely suspended oocysts of Cryptosporidium parvum and their suitability as DNA standards in qPCR
Although more modern methods are available, quantitative PCR (qPCR) is reproducible, sensitive and specific with instruments and expertise readily available in many laboratories. As such, the use of qPCR in Crypt...
Source: Parasites and Vectors - Category: Microbiology Authors: Ian D. Woolsey, Berit Blomstrand, Øivind Øines and Heidi L. Enemark Tags: Methodology Source Type: research