MicroRNA-210 protects against periodontitis through targeting HIF-3 α and inhibiting p38MAPK/NF-κB pathway.

MicroRNA-210 protects against periodontitis through targeting HIF-3α and inhibiting p38MAPK/NF-κB pathway. Artif Cells Nanomed Biotechnol. 2020 Dec;48(1):129-136 Authors: Jia S, Yang X, Yang X, Zhang F Abstract The aim of this study was to investigate the effects of miR-210 abnormal expression on Porphyromonas gingivalis lipopolysaccharide (LPS)-treated primary human periodontal ligament cells (PDLCs). The miR-210 level was identified in gingival tissues from patients with chronic periodontitis (CP) and healthy subjects as well as LPS-treated PDLCs by qRT-PCR. Cell viability, apoptotic cells, expression of proteins associated with apoptosis, and release of inflammatory factors in LPS-treated PDLCs were measured using MTT assay, flow cytometry assay, western blotting and ELISA, respectively. Effects of miR-210 abnormal expression on cell viability, cell apoptosis and inflammation factors in LPS-treated PDLCs were evaluated. Afterwards, the target gene of miR-210 was identified, and the involvement of p38MAPK/NF-κB pathway with the effects of miR-210 was finally studied. The miR-210 level was significantly down-regulated in gingival tissues from CP patients as well as LPS-treated PDLCs. LPS-induced decrease of cell viability, increase of apoptosis, and release of TNF-α, IL-1β, IL-6 and IL-8 were attenuated by miR-210 overexpression. We found that hypoxia-inducible factor (HIF)-3α was a target of miR-210, and HIF-3α overexpressio...
Source: Artificial Cells, Nanomedicine and Biotechnology - Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research