Viruses, Vol. 12, Pages 12: The Zebrafish Xenograft Platform —A Novel Tool for Modeling KSHV-Associated Diseases
Viruses, Vol. 12, Pages 12: The Zebrafish Xenograft Platform—A Novel Tool for Modeling KSHV-Associated Diseases
Viruses doi: 10.3390/v12010012
Authors:
Eric S. Pringle
Jaime Wertman
Nicole Melong
Andrew J. Coombs
Andrew L. Young
David O’Leary
Chansey Veinotte
Carolyn-Ann Robinson
Michael N. Ha
Graham Dellaire
Todd E. Druley
Craig McCormick
Jason N. Berman
Kaposi’s sarcoma associated-herpesvirus (KSHV, also known as human herpesvirus-8) is a gammaherpesvirus that establishes life-long infection in human B lymphocytes. KSHV infection is typically asymptomatic, but immunosuppression can predispose KSHV-infected individuals to primary effusion lymphoma (PEL); a malignancy driven by aberrant proliferation of latently infected B lymphocytes, and supported by pro-inflammatory cytokines and angiogenic factors produced by cells that succumb to lytic viral replication. Here, we report the development of the first in vivo model for a virally induced lymphoma in zebrafish, whereby KSHV-infected PEL tumor cells engraft and proliferate in the yolk sac of zebrafish larvae. Using a PEL cell line engineered to produce the viral lytic switch protein RTA in the presence of doxycycline, we demonstrate drug-inducible reactivation from KSHV latency in vivo, which enabled real-time observation and evaluation of latent and lytic phases of KSHV infection. In addition, we developed a sensitive droplet digital PCR method to monitor latent and lytic vira...
Source: Viruses - Category: Virology Authors: Eric S. Pringle Jaime Wertman Nicole Melong Andrew J. Coombs Andrew L. Young David O ’Leary Chansey Veinotte Carolyn-Ann Robinson Michael N. Ha Graham Dellaire Todd E. Druley Craig McCormick Jason N. Berman Tags: Article Source Type: research
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