Knockdown of MAPK14 inhibits the proliferation and migration of clear cell renal cell carcinoma by downregulating the expression of CDC25B

We examined the expression of mitogen ‐activated protein kinase 14 (MAPK14) and cell division cycle 25B (CDC25B) in clear cell renal cell carcinoma (ccRCC) and healthy tissue using the cancer genome atlas database and clinical samples. We affirm that our results reveal that phosphorylated MAPK14 (P‐MAPK14) and CDC25B are highly expr essed in ccRCC tissue, and that P‐MAPK14 binds to CDC25B, potentially stabilizing it. Additionally, MAPK14 knockdown inhibits the proliferation and migration of ccRCC cells, an effect that is partially reversed by CDC25B overexpression, suggesting that downregulation of MAPK14 and P‐MAPK14 inhib its the proliferation and migration of ccRCC by downregulating CDC25B. AbstractMitogen ‐activated protein kinase 14 (MAPK14), which plays an important role in DNA damage and repair, is activated by various environmental stress and proinflammatory cytokines. It is highly active in a variety of tumors, acting as a tumor promoter or suppressor, but its role in clear cell renal cell car cinoma (ccRCC) has not been elucidated. Cell division cycle 25B (CDC25B) is involved in cell cycle regulation and is highly expressed in many malignant tumors. The transcription levels of MAPK14 and CDC25B in 72 pairs of ccRCC and adjacent healthy tissues from the cancer genome atlas database and th e protein expression levels in 66 pairs of clinical samples were analyzed in this study. After MAPK14 was knocked down by small interfering RNA (siRNA), P‐MAPK14 and CDC...
Source: Cancer Medicine - Category: Cancer & Oncology Authors: Tags: ORIGINAL RESEARCH Source Type: research