An Immunoperoxidase Monolayer Assay (IPMA) for the detection of lumpy skin disease antibodies.

An Immunoperoxidase Monolayer Assay (IPMA) for the detection of lumpy skin disease antibodies.
J Virol Methods. 2019 Dec 11;:113800
Authors: Haegeman A, De Leeuw I, Mostin L, Van Campe W, Aerts L, Vastag M, De Clercq K
Abstract
During this study a new Immunoperoxidase Monolayer Assay (IPMA) was developed for the detection of antibodies against lumpy skin disease virus (LSDV) in an easy and low tech setting. Using two dilutions (1:50 and 1:300) in a duplicate format, the test was shown to be highly sensitive, specific and repeatable. In comparison to the VNT and a commercial ELISA, the LSDV-IPMA was able to detect the LSDV antibodies earlier in infected, vaccinated and vaccinated/infected animals. The assay is very flexible as it can be easily adapted for the detection of sheeppox or goatpox antibodies and it can be scaled-up to handle medium size sample sets by preparing the IPMA plates in advance. These plates are safe and can be handled in low biosafety level labs.
PMID: 31837373 [PubMed - as supplied by publisher]

Source: Journal of Virological Methods - December 10, 2019 Category: Virology Authors: Haegeman A, De Leeuw I, Mostin L, Van Campe W, Aerts L, Vastag M, De Clercq K Tags: J Virol Methods Source Type: research