High catalytic performance of Amano laccase wired to naphthylated multiwall carbon nanotubes

Publication date: Available online 14 December 2019Source: Biosensors and BioelectronicsAuthor(s): A. Ben Tahar, K. Żelechowska, J.F. Biernat, E. Paluszkiewicz, P. Cinquin, D. Martin, A. ZebdaAbstractThe direct electrical connection of laccase on the electrode surface is a key feature in the design of efficient and stable biocathodes. However, laccase can perform a direct electron transfer only when it is in the preferred orientation toward the electrode. Here we report the investigation of the orientation of laccase from white rot fungus on multi-walled carbon nanotube surface modified with a naphthalene group. Naphthylated multi wall carbon nanotubes were synthesized and the kinetics of laccase from white rot fungus adsorption and its direct electro-catalytic activity toward oxygen reduction was investigated by QCM and electrochemical techniques. Compared to pristine multi-walled carbon nanotubes laccase shows a high affinity to be adsorbed onto the surface of naphthylated carbon nanotubes at a very fast rate. The subsequent wiring to the naphthylated multi-walled carbon nanotubes is accompanied by a reorientation and arrangement of adsorbed laccase to create a composite biocathode that exhibits a high-performance for oxygen reduction by direct electron transfer with maximum current densities of 3 mA cm−2.
Source: Biosensors and Bioelectronics - Category: Biotechnology Source Type: research