MiRNA-101 targets TGF- βR1 to retard the progression of oral squamous cell carcinoma.

In this study, miR-101 expression was markedly downregulated in the OSCC cell lines and tissues. CCK-8, EdU, and colony formation assays showed that miR-101 inhibited the proliferation of OSCC cells. Flow cytometry and caspase-3 activity assays indicated that miR-101 induced OSCC cell apoptosis. Transwell assays demonstrated that this miRNA also repressed OSCC cell migration and invasion. Moreover, tube formation assay showed that miR-101 abated the pro-angiogenesis of OSCC cells. Dual luciferase reporter assay confirmed that miR-101 directly targeted TGF-βR1 in OSCC. Ectopic expression of TGF-βR1 counteracted the effects of miR-101 on the OSCC cell characteristics. Thus, miR-101 significantly abolished the proliferation, motility, and pro-angiogenesis of OSCC cells and induced their apoptosis by targeting TGF-βR1. These results imply the potential application of miR-101 in OSCC treatment. PMID: 31831099 [PubMed - as supplied by publisher]
Source: Oncology Research - Category: Cancer & Oncology Tags: Oncol Res Source Type: research