Genotyping of Acantamoeba spp. from rhisophere in Hungary.

In this study, 10 rhizosphere samples were collected from maize and alfalfa plants in experimental station at Institute of Genetics, Microbiology and Biotechnology, Szent István University. We detected Acanthamoeba based on the quantitative real-time PCR assay and sequence analysis of the 18S rRNA gene. All studied molecular biological methods are suitable for the detection of Acanthamoeba infection in humans. The quantitative real-time PCR-based methods are more sensitive, simple, and easy to perform; moreover, these are opening avenue to detect the effect of number of parasites on human disease. Acanthamoeba species were detected in five (5/10; 50%) samples. All Acanthamoeba strains belonged to T4 genotype, the main AK-related genotype worldwide. Our result confirmed Acanthamoeba strains in rhizosphere that should be considered as a potential health risk associated with human activities in the environment. PMID: 31833382 [PubMed - as supplied by publisher]
Source: Acta Microbiologica et Immunologica Hungarica - Category: Microbiology Authors: Tags: Acta Microbiol Immunol Hung Source Type: research

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ConclusionsFour subtypes (STs) of Blastocystis in human was identified in our study and subtype 3 was the most prevalent subtype. The common subtype (ST3) in this study was identical to the reports from other regions of Iran. For identification of the more subtypes of Blastocystis, comprehensive molecular studies with a large number of Blastocystis isolates are suggested.
Source: Clinical Epidemiology and Global Health - Category: Epidemiology Source Type: research
ConclusionsThe first study of its kind in Australia found T. gondii infection to be highly prevalent. Toxoplasma gondii infection has been neglected in Australian notifiable disease programs therefore Australian public health authorities should focus on improving education to raise awareness and commence longitudinal epidemiological data collection to supplement public health models targeting T. gondii transmission control.
Source: Clinical Epidemiology and Global Health - Category: Epidemiology Source Type: research
(eLife) Malaria parasites can sense a molecule produced by approaching immune cells and then use it to protect themselves from destruction, according to new findings published today in eLife.
Source: EurekAlert! - Infectious and Emerging Diseases - Category: Infectious Diseases Source Type: news
Contributors : Carlos Cordon-Obras ; Anna Barcons-Simon ; Christine Scheidig-Benatar ; Aurelie Cla ës ; Florent Dingli ; Damarys Loew ; Artur ScherfSeries Type : Genome binding/occupancy profiling by high throughput sequencingOrganism : Plasmodium falciparumHeterochromatin is essential in all eukaryotic systems to maintain genome integrity, long-term gene repression and to help chromosome segregation during mitosis. However, heterochromatin regions must be isolated to avoid its spreading over actively transcribed loci. Such function is accomplished by chromatin boundaries, DNA elements that block heterochromatin self-...
Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Genome binding/occupancy profiling by high throughput sequencing Plasmodium falciparum Source Type: research
Species of Acanthamoeba are facultative pathogens which can cause sight threatening Acanthamoeba keratitis and a rare but deadly brain infection, granulomatous amoebic encephalitis. Due to conversion of Acanthamo...
Source: Parasites and Vectors - Category: Microbiology Authors: Tags: Research Source Type: research
Acanthamoeba spp. can cause serious human infections, including Acanthamoeba keratitis, granulomatous amoebic encephalitis and cutaneous acanthamoebiasis. Cysteine biosynthesis and the L-serine metabolic pathway ...
Source: Parasites and Vectors - Category: Microbiology Authors: Tags: Research Source Type: research
Authors: Eroğlu F Abstract The free living amoebae cause various infections such as Acanthamoeba keratitis, granulomatous amoebic encephalitis, primer amoebic meningoencephalitis in humans and animals. The free living amoebae Acanthamoeba, Balamuthia mandriallis, Naegleria fowleria and Sappinia species that cause disease in humans have been isolated from many environmental materials until today. However, no isolation has been reported from the filters of the air conditions from the houses used for ventilation. The aim of this study was toinvestigate the existence of free living amoebae using molecular methods in t...
Source: Mikrobiyoloji Bulteni - Category: Microbiology Tags: Mikrobiyol Bul Source Type: research
o-Morales J Abstract Free-living amoebae of genus Acanthamoeba are opportunistic pathogens widely distributed in the environment, and are the causative agents of several humans' infections, such as Acanthamoeba keratitis, Granulomatous Amoebic Encephalitis and also disseminated infections. The existence of the cyst stage complicates Acanthamoeba therapy as it is highly resistant to antibiotics and physical agents. All these facts reinforced the necessity to find and develop an effective therapy against Acanthamoeba infections. In the present study, we are interested to several seaweeds species collected from the T...
Source: Experimental Parasitology - Category: Parasitology Authors: Tags: Exp Parasitol Source Type: research
d-Grillo T, Piñero JE, Lorenzo-Morales J Abstract Acanthamoeba is an opportunistic pathogen which is the causal agent of a sight-threatening ulceration of the cornea known as Acanthamoeba keratitis (AK) and, more rarely, an infection of the central nervous system called "granulomatous amoebic encephalitis" (GAE). The symptoms of AK are non-specific, and so it can be misdiagnosed as a viral, bacterial, or fungal keratitis. Furthermore, current therapeutic measures against AK are arduous, and show limited efficacy against the cyst stage of Acanthamoeba. 1H-Phenalen-1-one (PH) containing compounds ha...
Source: Experimental Parasitology - Category: Parasitology Authors: Tags: Exp Parasitol Source Type: research
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Source: The Journal of Eukaryotic Microbiology - Category: Microbiology Authors: Tags: Original Article Source Type: research
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