Prefrontal cortex inflammation and liver pathologies accompany cognitive and motor deficits following Western diet consumption in non-obese female mice

Publication date: 15 January 2020Source: Life Sciences, Volume 241Author(s): Ekaterina Veniaminova, Margarita Oplatchikova, Lucien Bettendorff, Elena Kotenkova, Alexander Lysko, Ekaterina Vasilevskaya, Allan V. Kalueff, Liliya Fedulova, Aleksei Umriukhin, Klaus-Peter Lesch, Daniel C. Anthony, Tatyana StrekalovaAbstractAimsThe high sugar and lipid content of the Western diet (WD) is associated with metabolic dysfunction, non-alcoholic steatohepatitis, and it is an established risk factor for neuropsychiatric disorders. Our previous studies reported negative effects of the WD on rodent emotionality, impulsivity, and sociability in adulthood. Here, we investigated the effect of the WD on motor coordination, novelty recognition, and affective behavior in mice as well as molecular and cellular endpoints in brain and peripheral tissues.Main methodsFemale C57BL/6 J mice were fed the WD for three weeks and were investigated for glucose tolerance, insulin resistance, liver steatosis, and changes in motor coordination, object recognition, and despair behavior in the swim test. Lipids and liver injury markers, including aspartate-transaminase, alanine-transaminase and urea were measured in blood. Serotonin transporter (SERT) expression, the density of Iba1-positive cells and concentration of malondialdehyde were measured in brain.Key findingsWD-fed mice exhibited impaired glucose tolerance and insulin resistance, a loss of motor coordination, deficits in novel object exploration and re...
Source: Life Sciences - Category: Biology Source Type: research