Structural and DNA Binding Properties of Mycobacterial Integration Host Factor mIHF

Publication date: Available online 14 December 2019Source: Journal of Structural BiologyAuthor(s): Nina T. Odermatt, Moreno Lelli, Torsten Herrmann, Luciano A. Abriata, Aleksandre Japaridze, Hubert Voilquin, Rajkumar Singh, Jérémie Piton, Lyndon Emsley, Giovanni Dietler, Stewart T. ColeAbstractIn bacteria, nucleoid associated proteins (NAPs) take part in active chromosome organization by supercoil management, three-dimensional DNA looping and direct transcriptional control. Mycobacterial integration host factor (mIHF, rv1388) is a NAP restricted to Actinobacteria and essential for survival of the human pathogen Mycobacterium tuberculosis. We show in vitro that DNA binding by mIHF strongly stabilizes the protein and increases its melting temperature. The structure obtained by Nuclear Magnetic Resonance (NMR) spectroscopy characterizes mIHF as a globular protein with a protruding alpha helix and a disordered N-terminus, similar to Streptomyces coelicolor IHF (sIHF). NMR revealed no residues of high flexibility, suggesting that mIHF is a rigid protein overall that does not undergo structural rearrangements. We show that mIHF only binds to double stranded DNA in solution, through two DNA binding sites (DBSs) similar to those identified in the x-ray structure of sIHF. According to Atomic Force Microscopy, mIHF is able to introduce left-handed loops of ca. 100 nm size (∼300 bp) in supercoiled cosmids, thereby unwinding and relaxing the DNA.
Source: Journal of Structural Biology - Category: Biology Source Type: research

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