Detergent-free extraction of a functional low-expressing GPCR from a human cell line

Publication date: Available online 13 December 2019Source: Biochimica et Biophysica Acta (BBA) - BiomembranesAuthor(s): Juan Francisco Bada Juarez, Juan C. Muñoz-García, Rosana Inácio dos Reis, Alistair Henry, David McMillan, Marco Kriek, Martyn Wood, Catherine Vandenplas, Zara Sands, Luis Castro, Richard Taylor, Anthony WattsAbstractDopamine receptors (DRs) are class A G-Protein Coupled Receptors (GPCRs) prevalent in the central nervous system (CNS). These receptors mediate physiological functions ranging from voluntary movement and reward recognition to hormonal regulation and hypertension. Drugs targeting dopaminergic neurotransmission have been employed to treat several neurological and psychiatric disorders, including Parkinson's disease, schizophrenia, Huntington's disease, attention deficit hyperactivity disorder (ADHD), and Tourette's syndrome. In vivo, incorporation of GPCRs into lipid membranes is known to be key to their biological function and, by inference, maintenance of their tertiary structure. A further significant challenge in the structural and biochemical characterization of human DRs is their low levels of expression in mammalian cells. Thus, the purification and enrichment of DRs whilst retaining their structural integrity and function is highly desirable for biophysical studies. A promising new approach is the use of styrene–maleic acid (SMA) copolymer to solubilize GPCRs directly in their native environment, to produce polyme...
Source: Biochimica et Biophysica Acta (BBA) Biomembranes - Category: Biochemistry Source Type: research

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Authors: Lancellotti P, De Pauw M, Claeys M Abstract Cardiovascular disease (CVD) and cancer represent the two main causes of death in industrialised countries. Both share common risk factors (diabetes, obesity, hypertension, diet, smoking, etc.). The associated timing of CVD and cancer onset is thus largely influenced by modifiable risk factors. Advances in cancer treatment have extended the lives of patients with cancer, but for some at the cost of adverse cardiovascular events. The rapidly growing number of patients surviving cancer, often in the setting of advanced age, new or pre-existing CV disease and risk f...
Source: Acta Cardiologica - Category: Cardiology Tags: Acta Cardiol Source Type: research
In conclusion, our diagnostic prediction model uses easily obtainable information to identify children at increased risk of high blood pressure, offering an opportunity for targeted screening. This model enables to detect a higher proportion of children with high blood pressure than a strategy based on child overweight only.
Source: Preventive Medicine - Category: International Medicine & Public Health Source Type: research
Angiotensin receptor blockers (ARBs), used in the treatment of hypertension, may modulate neurotransmission in the central nervous system. One previous very small case-control study had suggested that ARBs increase the odds of suicide. In a recent, large, ...
Source: SafetyLit - Category: International Medicine & Public Health Tags: Alcohol and Other Drugs Source Type: news
Conclusions: Tai Chi may be beneficial for improving balance and reducing falls in older people and those with Parkinson disease. Because of limitations and inconsistent conclusions, further rigorous, normative and comprehensive SRs are needed to provide robust evidence for definitive conclusions.
Source: Annals of Physical and Rehabilitation Medicine - Category: Rehabilitation Source Type: research
This study is the first GAM initiative in Europe.
Source: Social Science and Medicine - Category: Psychiatry & Psychology Source Type: research
ConclusionsOur preliminary findings show that baseline C4 levels predict clinical outcome at 1-year follow-up in patients with first episode psychosis.
Source: Brain, Behavior, and Immunity - Category: Neurology Source Type: research
This study examines neurocognitive outcomes of children with IF at ages 4 to 8 years treated at a single centre, along with relevant medical and demographic variables. Methods: Between 2012 and 2016, neurocognitive assessments were administered to 28 children receiving treatment in our IF rehabilitation program, and included measures of intelligence, academics, learning/memory, language, visual-motor integration, and fine-motor dexterity. DSM-IV/V criteria were used to diagnose Learning Disability, Intellectual Disability, and/or Attention Deficit/Hyperactivity Disorder. Results: Intellectual functioning ranged from ...
Source: Journal of Pediatric Gastroenterology and Nutrition - Category: Gastroenterology Tags: Original Articles: Gastroenterology Source Type: research
Publication date: Available online 22 January 2020Source: Best Practice &Research Clinical Endocrinology &MetabolismAuthor(s): Sheerazed Boulkroun, Fabio Luiz Fernandes-Rosa, Maria-Christina ZennaroAbstractPrimary aldosteronism (PA) is the most common form of secondary hypertension affecting 5% to 10% of patients with arterial hypertension. In PA, high blood pressure is associated with high aldosterone and low renin levels, and often hypokalemia. In a majority of cases, autonomous aldosterone production by the adrenal gland is caused by an aldosterone producing adenoma (APA) or bilateral adrenal hyperplasia (BAH). ...
Source: Best Practice and Research Clinical Endocrinology and Metabolism - Category: Endocrinology Source Type: research
Authors: Andrade C Abstract Angiotensin receptor blockers (ARBs), used in the treatment of hypertension, may modulate neurotransmission in the central nervous system. One previous very small case-control study had suggested that ARBs increase the odds of suicide. In a recent, large, population-based, nested case-control study conducted in Ontario, Canada, completed suicide was associated with significantly increased odds of an ARB prescription during the 100 days preceding the suicide; in this analysis, confounding by indication was reduced by comparing ARB exposure with angiotensin-converting enzyme inhibitor expo...
Source: Journal of Clinical Psychiatry - Category: Psychiatry Tags: J Clin Psychiatry Source Type: research
CONCLUSIONS: These findings suggest that careful clinical history taking to document specific neurodevelopmental features may be informative in screening for individuals with schizophrenia who are at higher risk of carrying known SCZ-associated CNVs. Identification of genomic disorders in these individuals is likely to have clinical benefits similar to those demonstrated for other neurodevelopmental disorders. PMID: 31964429 [PubMed - as supplied by publisher]
Source: The British Journal of Psychiatry for Mental Science - Category: Psychiatry Tags: Br J Psychiatry Source Type: research
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