miR-486-5p regulates cell proliferation and migration in breast cancer
In this study, the miR-86-5p was induced in breast cancer cells (MDA-MB-231) and the effect of miR-86-5p replacement on the expression level of VEGF, FOX, MMP3, SIRT1, and OLFM4 genes was investigated.Material and methodsThe miR-86-5p mature sequence was designed, synthesized and transfected into the MDA-MB-231 cells via jetPRIME® transfection reagent. The optimal concentration of miRNA was determined using qRT-PCR post-transfection. MTT assay was performed to evaluate the cell toxicity of miR-86-5p replacement. For finding the role of miR-86-5p in apoptosis, the annexin V/Propidium iodid (PI) assay was performed by flow cytometry analysis. A wound-healing assay (scratch assay) was performed to assess the effect of miR-86-5p on cell migration. The qRT-PCR method was used for measuring the expression level of miR-86-5p, VEGF, FOX, SIRT1, MMP3, and OLFM4.ResultsThe results showed that the optimal concentration of miRNA was 7.5 nM. The result of MTT and apoptosis assays revealed that miR-86-5p decreased cell viability and induced apoptosis. The wound healing assays showed that the miR-86-5p inhibited cell migration. Our results also showed that the miR-86-5p reduced VEGF, MMP3, OLFM4, and SIRT1 expression compared to the negative control cells. Thus, the miR-486 may act as a tumor suppressor and plays a vital role in cell apoptosis, cell growth and migration during breast cancer development. Our results showed that miR-86-5p could be a promising candidate to be used as a ther...
Source: Meta Gene - Category: Genetics & Stem Cells Source Type: research