Simple production of hydrophobin-fused domain III of dengue envelope protein and induction of neutralizing antibodies against the homotypic serotype of dengue virus.

Simple production of hydrophobin-fused domain III of dengue envelope protein and induction of neutralizing antibodies against the homotypic serotype of dengue virus. Biotechnol Lett. 2019 Dec 11;: Authors: Cerezo J, Targovnik AM, Smith ME, González Maglio D, Luppo VC, Morales MA, Miranda MV, Rodríguez Talou J Abstract Hydrophobin-fused domain III of dengue envelope proteins serotypes 1 and 2 were expressed in Rachiplusia nu larvae and purified by aqueous two-phase system. This biotechnological approach of hydrophobin-fused proteins, which allowed obtaining 97.7 µg/larva of fusion protein DomIII serotype 1 and 61.4 µg/larva of fusion protein DomIII serotype 2, represents an integrated strategy for simple production of recombinant antigens. Purified fusion proteins induced serotype-specific neutralizing antibodies without cross-reaction against other serotypes and arboviruses after mouse immunization. hydrophobin-fused domain III of dengue envelope protein could be a promising strategy for easy and low-cost production of components of a tetravalent sub-unit vaccine against dengue. PMID: 31828570 [PubMed - as supplied by publisher]
Source: Biotechnology Letters - Category: Biotechnology Authors: Tags: Biotechnol Lett Source Type: research

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Source: Frontiers in Immunology - Category: Allergy & Immunology Source Type: research
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Source: JAMA - Category: General Medicine Source Type: research
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Source: Antiviral Therapy - Category: Virology Source Type: research
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Source: Antiviral Research - Category: Virology Authors: Tags: Antiviral Res Source Type: research
PMID: 31909484 [PubMed - as supplied by publisher]
Source: Med J Aust - Category: General Medicine Authors: Tags: Med J Aust Source Type: research
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