Structural characterization and antiviral activity of two fucoidans from the brown algae Sargassum henslowianum.

Structural characterization and antiviral activity of two fucoidans from the brown algae Sargassum henslowianum. Carbohydr Polym. 2020 Feb 01;229:115487 Authors: Sun QL, Li Y, Ni LQ, Li YX, Cui YS, Jiang SL, Xie EY, Du J, Deng F, Dong CX Abstract Purified fucoidans SHAP-1 and SHAP-2 with apparent molecular weights of 6.55 × 105 and 5.89 × 105, respectively, were isolated from Sargassum henslowianum by ion-exchange and gel-filtration column chromatography. They are both composed of fucose and galactose at a ratio of around 3:1 and 31.9% sulfate. The backbone of two fucoidans consists of α-(1→3)-linked L-Fucp residues which are mainly sulfated on the C-2 and C-4 positions. Side chains composed of terminally linked α-L-Fucp and α-D-Galp residues, and (1→2)-, (1→6)-, and (1→2,6)-linked β-D-Galp residues attach mainly at O-4 position of backbone residues. Antiviral test showed that the IC50 values of SHAP-1 and SHAP-2 against HSV-1 were estimated to be 0.89 and 0.82 μg/mL by plaque reduction assay, respectively, whereas both as low as 0.48 μg/mL against HSV-2. The antiviral mechanism of the fucoidans might be at least through blocking HSV-2 virion adsorption to host cells. These results suggest that the fucoidans have potential clinical applications. PMID: 31826428 [PubMed - in process]
Source: Herpes - Category: Infectious Diseases Authors: Tags: Carbohydr Polym Source Type: research