Advances in the detection of virulence genes of Staphylococcus aureus originate from food

Publication date: Available online 12 December 2019Source: Food Science and Human WellnessAuthor(s): Yingying Zhao, Dandan Xia, Panpan Ma, Xiaofeng Gao, Wenyi Kang, Jinfeng WeiAbstractHuman health is threatened by foodborne illness and Staphylococcus aureus is a common foodborne pathogenic bacteria. It can cause food poisoning when we are infected, therefore, it is necessary to detect pathogenic bacteria. The virulence genes and detection methods of S. aureus are summarized from literatures. Traditional detection methods are simple but need bacteria enrichment and to prolong detection time. Immunological technology has high specificity, but false positive results are easy to occur in its detection. In recent years, molecular biology methods have developed rapidly, and various PCR techniques have been applied to detect foodborne pathogens. Real-time fluorescent quantitative PCR is more effective than ordinary PCR, while price is expensive when use it, the scope of use is limited. LAMP is a flexible detection method, biosensor and flow cytometry are fast but the cost of detection is high and the price is relatively expensive.
Source: Food Science and Human Wellness - Category: Food Science Source Type: research

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In this study, six enterotoxigenic S. aureus strains were characterised using a combination of molecular, phenotypical and computational methods. The genomes were analysed for the presence of virulence factors (VFs), where we identified 110 genes and classified them into five categories: adherence (n = 31), exoenzymes (n = 28), genes involved in host immune system evasion (n = 7); iron uptake regulatory system (n = 8); secretion machinery factors and toxins’ genes (n = 36), and 39 genes coding for transcriptional regulators related to staphylococcal VFs. Each group of VFs revealed correlations among the six enter...
Source: Genes - Category: Genetics & Stem Cells Authors: Tags: Article Source Type: research
Authors: Deng Y, Liu Y, Jiang Z, Wang J, Zhang Q, Qian Y, Yuan Y, Zhou X, Fan G, Li Y Abstract Bacillus cereus (B. cereus) and Staphylococcus aureus (S. aureus) are major human food-borne pathogens that may produce a variety of toxins and cause diarrhea, food poisoning, and even death. In order to monitor and prevent the spread of these pathogens, a multiplex loop-mediated isothermal amplification (multi-LAMP) assay was developed to simultaneously and rapidly detect B. cereus and S. aureus. The sensitivity and specificity of the loop-mediated isothermal amplification (LAMP) reactions were determined via electrophor...
Source: BioScience Trends - Category: Biomedical Science Tags: Biosci Trends Source Type: research
Foods may potentially serve as vehicles for the transmission of antimicrobial-resistant variants of Staphylococcus aureus that are important in a human clinical context. Further, retail food products can be a cause of staphylococcal food poisoning. For these reasons and to account for source attribution and risk assessment, detailed information on the population structure, resistance, and virulence profiles of S. aureus originating from retail food products is necessary. In the current study, whole-genome sequences from 88 S. aureus isolates were subjected to bioinformatics analyses in relation to sequence types, antimicro...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
Staphylococcus aureus is a bacterial pathogen that causes food poisoning, various infections, and sepsis. Effective strategies and new drugs are needed to control S. aureus associated infections due to the emergence and rapid dissemination of antibiotic resistance. In the present study, the antibacterial activity, potential mode of action, and applications of flavonoids from licorice were investigated. Here, we showed that glabrol, licochalcone A, licochalcone C, and licochalcone E displayed high efficiency against methicillin-resistant Staphylococcus aureus (MRSA). Glabrol, licochalcone A, licochalcone C, and licochalcone...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
In this study, we have suggested that LysSAP8 could be a potent alternative as a biocontrol agent that can be used to combat MRSA. PMID: 31635440 [PubMed - as supplied by publisher]
Source: Journal of Microbiology and Biotechnology - Category: Biotechnology Authors: Tags: J Microbiol Biotechnol Source Type: research
Publication date: January 2020Source: LWT, Volume 117Author(s): Darla Silveira Volcan Maia, Louise Haubert, Isabela Schneid Kroning, Kauana dos Santos Soares, Thaís Larré Oliveira, Wladimir Padilha da SilvaAbstractThe aims of this study were to verify the ability of S. aureus isolates from food poisoning outbreaks to form biofilm, and evaluate the susceptibility of these isolates to Butia odorata extract (BOE) and its effect on S. aureus biofilm. All 13 S. aureus isolates formed biofilm on polystyrene; and 84.6% (11/13) carried icaA and icaB genes, 69.2% (9/13) the icaD gene, and 61.5% (8/13) the icaC gene. A...
Source: LWT Food Science and Technology - Category: Food Science Source Type: research
Publication date: Available online 18 September 2019Source: Food BioscienceAuthor(s): Sarengaowa, Wenzhong Hu, Ke Feng, Zhilong Xiu, Aili Jiang, Ying LaoAbstractFresh-cut cantaloupe (Cucumis melo L.) is a popular food. However, the presence of foodborne pathogen on the cut surfaces leads to both spoilage and food poisoning of fresh-cut cantaloupe. The antibacterial effectiveness of an alginate-based edible coating (EC) containing thyme oil was evaluated against foodborne pathogens on fresh-cut cantaloupe samples. Cantaloupe samples were treated with alginate-based EC and alginate-based EC containing different concentration...
Source: Food Bioscience - Category: Food Science Source Type: research
Conclusion: The present study exhibited favorable antagonistic effects of S. cerevisiae S3, as a probiotic yeast, on MSSA and MRSA strains. Based on the findings of this study, the compounds produced by this yeast can be used to control S. aureus infections; however, further similar studies should be conducted to confirm the findings of the present study. PMID: 31523409 [PubMed]
Source: Iranian Journal of Microbiology - Category: Microbiology Tags: Iran J Microbiol Source Type: research
In this study, we evaluated the microbiota of perilla leaves harvested in South Korea using Illumina MiSeq sequencing of the 16S rRNA gene. In total, 2,743,003 sequencing reads were obtained, and 92–437 operational taxonomic units were observed in all samples. Bacterial loads were quantified, and the diversity indices were compared. Differences in the microbiota among sampling times and regions were also investigated. Proteobacteria and Firmicutes were predominant phyla at both times. At the class level, the bacterial communities were composed primarily of Alphaproteobacteria, Bacilli, and Gammaproteobacteria. Divers...
Source: Food Research International - Category: Food Science Source Type: research
Publication date: January 2020Source: Food Control, Volume 107Author(s): Angelo Colagiorgi, Rossella Festa, Pierluigi A. Di Ciccio, Marta Gogliettino, Marco Balestrieri, Gianna Palmieri, Aniello Anastasio, Adriana IanieriAbstractStaphylococcal food poisoning – caused by certain enterotoxigenic staphylococci – is one of the most common worldwide foodborne diseases. In this context, bacterial biofilms on food processing plan surfaces can represent critical sources of contamination, being more resistant to cleaning and disinfection procedures. Therefore, it is important to prevent and control biofilm formation in ...
Source: Food Control - Category: Food Science Source Type: research
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