Potent virucidal activity against Flaviviridae of a group IIA phospholipase A2 isolated from the venom of Bothrops asper

Publication date: Available online 12 December 2019Source: BiologicalsAuthor(s): Hebleen Brenes, Gilbert D. Loría, Bruno LomonteAbstractSecreted phospholipase A2 (sPLA2) molecules are small, calcium-dependent enzymes involved in many biological processes. Viperid venoms possess gIIA sPLA2s and sPLA2-like proteins, both having homology to human gIIA sPLA2, an innate immunity enzyme. We evaluated the antiviral action of Mt–I (catalytically-active sPLA2) and Mt-II (catalytically-inactive variant) isolated from the venom of Bothrops asper, against a diverse group of viruses. Yellow Fever and Dengue (enveloped) viruses were highly susceptible to inactivation by the snake proteins, in contrast to Sabin (non-enveloped; Polio vaccine strain), and Influenza A, Herpes simplex 1 and 2, and Vesicular Stomatitis (enveloped) viruses. Titration of the antiviral effect against Dengue virus revealed Mt–I to be highly potent (IC50 0.5–2 ng/mL), whereas Mt-II was 1000-fold weaker. This large difference suggested a requirement for PLA2 activity, which was confirmed by chemical inactivation of Mt–I. A synthetic peptide representing the membrane-disrupting region of Mt-II, previously shown to have bactericidal effect, lacked antiviral action, suggesting that the weak virucidal effect observed for Mt-II is likely caused by contamination with traces of Mt–I. On the other hand, Mt–I was demonstrated to act by a direct virucidal mechanism prior to infect...
Source: Biologicals - Category: Biology Source Type: research

Related Links:

This study also shortlists the essential genes of N. gonorrhoeae that could be used to target Neisseria. We identified a molecule/drug which can be used to target essential protein DapD (succinyltransferase).
Source: Journal of Theoretical Biology - Category: Biology Source Type: research
In this study, we designed a tandem sequence of CpG ODN synthesized in plasmid pcDNA 3.1, and an inactivated Vibrio anguillarum vaccine developed in our previous work was chosen for determining the efficiency of the CpG-riched plasmids (pCpG) as an adjuvant. Results showed that pCpG we designed can offer higher immunoprotection with the vaccine. Interestingly, even below the minimum immune dosage of the vaccine, a high RPS of 84% was observed once the vaccine was administrated with the pCpG. Serum specific antibody titer, superoxide dismutase and total protein were enhanced and some immune genes related to both innate and ...
Source: Fish and Shellfish Immunology - Category: Biology Source Type: research
Background: One ‑visit apexification is a treatment of choice in necrotic immature open apexteeth. Calcium silicate base materials are suitable for this method. The purpose of this study wasto investigate and compare the sealing efficiency of Biodentine, mineral trioxide aggregate (MTA)ProRoot, and calcium ‑enriched mixture (CEM) cement orthograde apical plug using bacterialleakage method.Materials and Methods: In this in vitro study a total of 70 extracted maxillary incisors werecleaned and shaped. A 1.1 ‑mm standardized artificially open apex was created in all samples.The teeth were randomly divided into three exp...
Source: Dental Research Journal - Category: Dentistry Source Type: research
Conclusion: The present study revealed that no statistically significant differenceswere observed between the two apex locators after Ca(OH)2 paste removal with differentirrigants.
Source: Dental Research Journal - Category: Dentistry Source Type: research
Contributors : Ting Xie ; Dianhua JiangSeries Type : Expression profiling by arrayOrganism : Mus musculusThe successful repair and renewal of alveolar epithelial cells are critical steps in prohibiting the accumulation of myofibroblasts and deposition of extracellular matrix in pulmonary fibrogenesis. MicroRNAs (miRNAs) are multi-focal regulators involved in the lung repair process. The contribution of miRNAs to epithelial maintenance and renewal is incompletely understood. We provide evidence that miR-29c on type 2 alveolar epithelial cells (AEC2s) are important for inhibiting AEC2 apoptosis and promoting AEC2 renewal, th...
Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Expression profiling by array Mus musculus Source Type: research
This study explored and proved the good efficiency of β-glucan or anisodamine as immersion immune adjuvant and also provided reference for improving the efficiency of immersion immunity.
Source: Fish and Shellfish Immunology - Category: Biology Source Type: research
In this study for the synthesis of zinc and chromium co-doped calcium hydroxyapatite (Ca10(PO4)6(OH)2:Zn,Cr; CHAp:Zn,Cr) an aqueous sol-gel processing has been used. The substitution effects on the sinterability, phase purity and morphological properties of CHAp:Zn, Cr were investigated in the present work. The proposed synthesis route is suitable for the preparation of monophasic calcium hydroxyapatite doped with zinc and chromium up to 7%. Single-phase CHAp:Zn, Cr samples were immersed into simulated body fluid (SBF) solution for one month. The formation of low crystalline CHAp in SBF possibly via dissolution-precipitati...
Source: Journal of Solid State Chemistry - Category: Chemistry Source Type: research
A new detection method rolled out last month looks for traces of the human papillomavirus (HPV), which cause nearly all cervical tumours.
Source: the Mail online | Health - Category: Consumer Health News Source Type: news
Publication date: Available online 18 January 2020Source: Toxicology ReportsAuthor(s): Afaf M. Weli, Hanady S. Al-Saadi, Rouqaya S. Al-Fudhaili, Amzad Hossain, Zabida Binti Putit, Mahmood K. JasimAbstractMedicinal plant as herbal medicine is widely used to cure infectious diseases. Rubus fruticosus L (R. fruticosus) has been studied for its antimicrobial and cytotoxic activities. Different polarity leave extracts were prepared by using hexane, chloroform, ethyl acetate and hydro alcoholic solvents. Agar diffusion method has been used to assess the antibacterial activity against two gram-positive Enterococcus faecalis and S...
Source: Toxicology Reports - Category: Toxicology Source Type: research
nte B Abstract Secreted phospholipase A2 (sPLA2) molecules are small, calcium-dependent enzymes involved in many biological processes. Viperid venoms possess gIIA sPLA2s and sPLA2-like proteins, both having homology to human gIIA sPLA2, an innate immunity enzyme. We evaluated the antiviral action of Mt-I (catalytically-active sPLA2) and Mt-II (catalytically-inactive variant) isolated from the venom of Bothrops asper, against a diverse group of viruses. Yellow Fever and Dengue (enveloped) viruses were highly susceptible to inactivation by the snake proteins, in contrast to Sabin (non-enveloped; Polio vaccine strain...
Source: Biologicals : Journal of the International Association of Biological Standardization - Category: Biotechnology Authors: Tags: Biologicals Source Type: research
More News: Biology | Calcium | Chemistry | Cold Sores | Dengue Fever | Herpes | Influenza | Influenza Vaccine | Polio | Polio Vaccine | Vaccines | Yellow Fever | Yellow Fever Vaccination