MicroRNA profiling of gastric content from breast-fed and formula-fed infants to estimate last feeding: a pilot study

AbstractRecently, we were consulted about a challenging case, where an infant died by poisoning and the drug-dependent mother insisted that she unintentionally gave the toxic drug through breast milk. Accordingly, we investigated the utility of immunoblotting and microRNA (miRNA) profiling of the infant ’s gastric content (GC) to differentiate between breast-feeding and formula-feeding. As a pilot study, we sampled the GC from breast-fed (GCB) and formula-fed (GCF) infants, as well as gastric juice (GJ) from fasted adults at autopsy. Breast milk (BM) samples were collected from volunteers within 1 year post-delivery. By immunoblotting, lactoferrin and gross cystic disease fluid protein (GCDEP) were clearly detected in BM, but could not be detected in GCB. Similarly, β-lactoglobulin was detected in formula milk, but could not be detected in GCF. Meanwhile, miRNA sequencing revealed that th e miRNA expression profile of GCB was closer to BM than GCF and GJ. Especially, miR-151a and miR-186 were more abundant in BM and GCB than in GCF and GJ. Our study is the first to elucidate the human GJ miRNA profile and demonstrate the possibility that miR-151a and miR-186 in GC may be the biomarke r of breast-feeding.
Source: International Journal of Legal Medicine - Category: Medical Law Source Type: research

Related Links:

Publication date: Available online 22 January 2020Source: Mutation Research/Genetic Toxicology and Environmental MutagenesisAuthor(s): Donna Goodenow, Faith Emmanuel, Chase Berman, Mark Sahyouni, Christine RichardsonAbstractBioflavonoids have a similar chemical structure to etoposide, the well-characterized topoisomerase II (Top2) poison, and evidence shows that they also induce DNA double-strand breaks (DSBs) and promote genome rearrangements. The purpose of this study was to determine the kinetics of bioflavonoid-induced DSB appearance and repair, and their dependence on Top2. Cells were exposed to bioflavonoids individu...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - Category: Genetics & Stem Cells Source Type: research
Authors: Kondo K, Sakata K, Kato R, Sugano Y, Takeuchi S, Sato M Abstract Qualitative real-time PCR method for three poisonous Entoloma rhodopolium-related species in Japan was established using specific primers and FAM, VIC, Texas Red, Cy5-labeled probes. The use of multicolor probes can extend the method to simultaneous detection of different targets. Standard plasmids were constructed as reference materials. Designed primers and probes in the method detect only a target species, and the detection limit was 12.5 copies or below. This indicates it is highly specific and sensitive enough to detect the poisonous mus...
Source: Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan - Category: Food Science Tags: Shokuhin Eiseigaku Zasshi Source Type: research
NEW HAVEN, Conn. - A lead-paint poisoning plaintiff on Jan. 9 moved jointly with the city of New Haven in Connecticut state court seeking an extension of time for the plaintiff to reply to the city's motion to dismiss in order to review the case and explore the possibility of a settlement (Nichelle Hobby, et al. v. City of New Haven, No. NHH-CV-19-5003875-S, Conn. Super., New Haven Jud. Dist.).
Source: LexisNexis® Mealey's™ Emerging Toxic Torts Legal News - Category: Medical Law Source Type: news
PURPOSE: To explore neuropathologic mechanisms in functional brain regions in patients with delayed encephalopathy after carbon monoxide poisoning (DEACMP) from the perspective of the brain network nodes by resting-state functional magnetic resonance imagi...
Source: SafetyLit - Category: International Medicine & Public Health Tags: Economics of Injury and Safety, PTSD, Injury Outcomes Source Type: news
[Daily Maverick] Australians may not know and will be unhappy to discover that South African waters are far from pristine, with toxic runoff from factories and farms entering coastal waters. Local sharks are apex predators and, as bioaccumulators, they retain heavy metals such as mercury and arsenic.
Source: AllAfrica News: Health and Medicine - Category: African Health Source Type: news
CONCLUSIONS Despite the high coverage of the Family Health Strategy, occupational risk and its consequences have not been detected by health services, which do not seem oriented to primary care, even lacking their essential attributes. There is a need for immediate and effective adaptation of public policies regarding the health of rural workers, with adequate training of teams and review of the portfolio of PHC services offered.ABSTRACT OBJETIVO Avaliar os atributos da aten ção primária à saúde (APS) na assistência à saúde de trabalhadores rurais; analisar condi&ccedi...
Source: Revista de Saude Publica - Category: International Medicine & Public Health Source Type: research
Publication date: Available online 20 January 2020Source: Toxicology ReportsAuthor(s): Hiroshi Kinoshita, Hülya Türkan, Slavica Vucinic, Shahab Naqvi, Rafik Bedair, Ramin Rezaee, Aristides TsatsakisAbstractCarbon monoxide (CO) is the leading cause of poisoning deaths in many countries, including Japan. Annually, CO poisoning claims about 2000-5000 lives in Japan, which is over half of the total number of poisoning deaths. This paper discusses the physicochemical properties of CO and the toxicological evaluation of CO poisoning.
Source: Toxicology Reports - Category: Toxicology Source Type: research
Gambierdiscus and Fukuyoa species have been identified in Aotearoa/New Zealand ’s coastal waters and G. polynesiensis, a known producer of ciguatoxins, has been isolated from Rangitāhua/Kermadec Islands (a New Zealand territory). The warming of the T...
Source: SafetyLit - Category: International Medicine & Public Health Tags: Home and Consumer Product Safety Source Type: news
Misuse of prescription opioids is a leading cause of premature death in the United States. We use state government administrative data and machine learning methods to examine whether the risk of future opioid dependence, abuse, or poisoning can be predicte...
Source: SafetyLit - Category: International Medicine & Public Health Tags: Alcohol and Other Drugs Source Type: news
Industrial release of mercury into the local Minamata environment with consequent poisoning of local communities through contaminated fish and shellfish consumption is considered the classic case of environmental mercury poisoning. However, the mercury spe...
Source: SafetyLit - Category: International Medicine & Public Health Tags: Disaster Preparedness Source Type: news
More News: Breastfed | Gastroenterology | Legislation | Medical Law | Milk | Poisoning | Study | Toxicology