Molecules, Vol. 24, Pages 4547: Coagulant Effects and Mechanism of Schefflera heptaphylla (L.) Frodin

Molecules, Vol. 24, Pages 4547: Coagulant Effects and Mechanism of Schefflera heptaphylla (L.) Frodin Molecules doi: 10.3390/molecules24244547 Authors: Xuqiang Liu Jing Dong Qiongxin Liang Hui-min David Wang Zhenhua Liu Ruian Xu Wenyi Kang Schefflera heptaphylla (L.) Frodin, are commonly used in anti-inflammatory, analgesic, traumatic bleeding and hemostasisas. In this paper, the coagulation effect of the ethanol extract (Set), ethyl acetate phase (Sea) and n-butanol phase (Sbu) was evaluated by prothrombin time (PT), activated partial thromboplastin time (APTT), thrombin time (TT) and fibrinogen content (FIB) assays in vitro. Then, Three main lupanine triterpenes (compounds A–C) were isolated and identified from Sea and Sbu by a bioassay-guided method and their structure were identified as 3α-Hydroxy-lup-20(29)-ene-23, 28-dioic acid, betulinic acid 3-O-sulfate and 3α-Hydroxy-lup-20(29)-ene-23, 28-dioic acid 28-O-(α-l-rhamnopyranosyl(1→4)-O-β-d-glucopyranosyl(1→6))-β-d-glucopyranoside) by spectroscopic data analysis. Among of them, compound B was confirmed to have significant coagulant effect in vitro. Furthermore, the pro-coagulation mechanism of S. heptaphylla extracts and compound B were investigated by measuring whole blood viscosity (WBV), plasma viscosity (PV), erythrocyte sedimentetion rate (ESR), pack cell volume (PCV), APTT, PT, TT, and FIB ...
Source: Molecules - Category: Chemistry Authors: Tags: Article Source Type: research