Rate of false positive reactions in 11 M. gallisepticum and M. synoviae serological tests in samples obtained from SPF birds inoculated with heterologous mycoplasma species.

In this study the performance of a current available Mg and Ms RPA test, three Mg, three Ms and three Mg/Ms combination ELISAs were evaluated on SPF sera that were obtained from day (D) 0-28 after M. gallinarum, M. imitans or M. gallinaceum inoculation, after sham inoculation and without inoculation. Trachea swabs for mycoplasma culture were obtained before inoculation (D0), 7 and 28 days post inoculation (d.p.i.) in all groups except the sham inoculated group. The different mycoplasma species colonized well. In the early stage after inoculation(7-14 d.p.i.) with heterologous mycoplasma species, the specificity varied from 85% to 100% in the Mg RPA test and from 70% to 85% in the Ms RPA test. The specificity of both Mg and Ms RPA test was 100% in the sham inoculated samples and ruled out effect of sham medium. In the late stage (21-28 d.p.i.) specificity was 100% for both RPA tests. The test specificity was 100% for 7 ELISAs except for two combination ELISAs where a specificity of 95% was found in the late stage after inoculation. However this was not significantly different from the specificity of all other tests in the chronic phase of these groups. These results show that it is not advisable to establish Mg and Ms seromonitoring programmes on the Mg and Ms RPA test alone as other mycoplasma species frequently occur in poultry.
PMID: 31818125 [PubMed - as supplied by publisher]

Source: Avian Pathology - December 9, 2019 Category: Pathology Authors: Feberwee A, Dijkman R, Wiegel J, Veen CT, Bataille H, Bouwstra R, de Wit S Tags: Avian Pathol Source Type: research