A novel high-resolution melting analysis-based method for Salmonella genotyping

Publication date: Available online 11 December 2019Source: Journal of Microbiological MethodsAuthor(s): Miaomiao Hu, Dong Yang, Xiaoli Wu, Meng Luo, Feng XuAbstractTo establish a simple and rapid high-resolution melting curve (HRM) method, 5 different strains of Salmonella were identified by adding DNA denaturants at different concentrations into the HRM system to change the characteristics of DNA melting and to obtain different Tm (dissolving temperature) values of DNA from different target bacteria. When the concentration of n-butanol was 7% (v/v), the Tm value of the melting curve of the 5 strains changed from 89 °C to 80.5 °C, 81.5 °C, 79.5 °C, 81.0 °C and 82.5 °C, respectively. The sensitivity and specificity of the proposed method were both over 90% in the detection of 270 spiked milk powder samples. In summary, the proposed method in this study has potential for application to food safety and epidemiological research on Salmonella infection.
Source: Journal of Microbiological Methods - Category: Microbiology Source Type: research

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Bravo Produce Inc de San Ysidro, est á retirando Maradol Papaya, cultivadas por Productores y Exportadores de Carica Papaya de Tecoman y Costa Alegre SPR de RL y empacadas por Frutas Selectas de Tijuana, S. de R.L. de C.V., como manera preventiva, debido a que dicho producto ha probado positivo para Salmonella, es de suma importan cia mencionar que dicha bacteria puede afectar a niños, ancianos y personas con sistemas inmunológicos débiles. Los síntomas a experimentar son fiebre, diarrea, náusea, vómito y dolor abdominal. En raras circunstancias, la infección por Salm...
Source: FDA Food Safety - Category: Food Science Authors: Source Type: alerts
Analyst, 2020, Accepted Manuscript DOI: 10.1039/C9AN02011J, PaperLinlin Zhuang, Jiansen Gong, Yongxin Ji, Peilong Tian, Fei Kong, Huiyuan Bai, Ning Gu, Yu Zhang Salmonella spp. are zoonotic pathogens of substantial public health concern. To enable detection in the field or instrument-free conditions, we developed a rapid and robust lateral flow fluorescent immunoassay based... The content of this RSS Feed (c) The Royal Society of Chemistry
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Publication date: January 2020Source: Journal of Comparative Pathology, Volume 174Author(s): N. de Bruijn, C. Ter Veen, A. Heuvelink, A. Feberwee
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Recently nanomaterials have received substantial attention in biotechnology areas for their innovative properties in physical and chemical function. One of the most arrestive properties of nanomaterials that has been reported is their bacteriostatic activity. Our previous research found that Fe3O4 magnetic nanoparticles (Fe3O4-NPs) could effectively reduce the viability of intracellular Salmonella Enteritidis in chicken cells. There is an essential need to explore whether the bacteriostatic activity of Fe3O4-NPs is available in vivo. As an extension of this research, we conducted the present study to investigate the potent...
Source: Frontiers in Physiology - Category: Physiology Source Type: research
Mucosal Immunology, Published online: 17 January 2020; doi:10.1038/s41385-019-0247-0▓
Source: Mucosal Immunology - Category: Allergy & Immunology Authors: Source Type: research
Publication date: 1 March 2020Source: Materials Chemistry and Physics, Volume 243Author(s): Samir Bouchareb, Rachida Doufnoune, Farid Riahi, Hafsa Cherif-Silini, Lassaad BelbahriAbstractGO-Ag nanohybrids have attracted tremendous attention thanks to their several potential properties. In this research work, a new approach was adopted, where Triphenylphosphine (PPh3) was used as a linkage to decorate the surface of Graphene oxide (GO) nanosheets by Silver nanoparticles (AgNPs) via a simple method. The Fourier transform infrared spectroscopy (FTIR), Ultraviolet–visible spectroscopy (UV–Vis), X-ray diffraction ana...
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Publication date: Available online 15 January 2020Source: Microbial PathogenesisAuthor(s): Dan Xiao, Guilin Yang, Zhenhua Wang, Abdul Khalique, Zhanwei Zhu, Lvchen Xiong, Jianzhen Li, Xinru Yuan, Xueqin Ni, Dong Zeng, Dongmei Zhang, Kangcheng PanAbstractTo investigate the effects of Bacillus methylotrophicus SY200 on Salmonella typhimurium (STM) infection in mice, a total of 36 three-week-old male mice were selected and randomly divided into 3 equal groups (N = 12). Group A and group B were fed with basal diet while group C was fed the basal diet supplemented with 0.1% (w/w) B. methylotrophicus SY200 during the 21 day ...
Source: Microbial Pathogenesis - Category: Infectious Diseases Source Type: research
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Source: Molecular and Cellular Probes - Category: Molecular Biology Source Type: research
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Publication date: Available online 15 January 2020Source: Journal of Photochemistry and Photobiology A: ChemistryAuthor(s): Pablo J. Gonçalves, Fabio C. Bezzerra, Amanda V. Teles, Lucas B. Menezes, Kamilla M. Alves, Lais Alonso, Antonio Alonso, Maria A. Andrade, Iouri E. Borissevitch, Guilherme R.L. de Souza, Bernardo A. IglesiasAbstractIn the last years, antimicrobial photodynamic therapy (aPDT) has been considered as one important alternative to be used in antimicrobial treatments. In the present work we report the first photoinactivation of Salmonella enterica serovar Typhimurium (Salmonella Typhimurium) by catio...
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