Automatic Microscopic Image Analysis by Moving Window Local Fourier Transform and Machine Learning

Publication date: Available online 11 December 2019Source: MicronAuthor(s): Benedykt R. Jany, Arkadiusz Janas, Franciszek KrokAbstractAnalysis of microscope images is a tedious work which requires patience and time, usually done manually by the microscopist after data collection. The results obtained in such a way might be biased by the human who performed the analysis. Here we introduce an approach of automatic image analysis, which is based on locally applied Fourier Transform and Machine Learning methods. In this approach, a whole image is scanned by a local moving window with defined size and the 2D Fourier Transform is calculated for each window. Then, all the Local Fourier Transforms are fed into Machine Learning processing. Firstly, a number of components in the data is estimated from Principal Component Analysis (PCA) Scree Plot performed on the data. Secondly, the data are decomposed blindly by Non-Negative Matrix Factorization (NMF) into interpretable spatial maps (loadings) and corresponding Fourier Transforms (factors). As a result, the microscopic image is analyzed and the features on the image are automatically discovered, based on the local changes in Fourier Transform, without human bias. The user selects only a size and movement of the scanning local window which defines the final analysis resolution. This automatic approach was successfully applied to analysis of various microscopic images with and without local periodicity i.e. atomically resolved High Angl...
Source: Micron - Category: Biology Source Type: research