The extraradical proteins of Rhizophagus irregularis: a shotgun proteomics approach

Publication date: Available online 10 December 2019Source: Fungal BiologyAuthor(s): Chelsea L. Murphy, Noha H. Youssef, Steve Hartson, Mostafa S. ElshahedAbstractArbuscular Mycorrhizal fungi (AMF, Glomeromycota) form obligate symbiotic associations with the roots of most terrestrial plants. Our understanding of the molecular mechanisms enabling AMF propagation and AMF-host interaction is currently incomplete. Analysis of AMF proteomes could yield important insights and generate hypotheses on the nature and mechanism of AMF-plant symbiosis. Here, we examined the extraradical mycelium proteomic profile of the arbuscular mycorrhizal fungus Rhizophagus irregularis grown on Ri T-DNA transformed Chicory roots in a root organ culture setting. Our analysis detected 529 different peptides that mapped to 474 translated proteins in the Rhizophagus irregularis genome. R. irregularis proteome was characterized by a high proportion of proteins (9.9 % of total, 21.4% of proteins with functional prediction) mediating a wide range of signal transduction processes, e.g. Rho1 and Bmh2, Ca-signaling (calmodulin, and Ca channel protein), mTOR signaling (MAP3K7, and MAPKAP1), and phosphatidate signaling (phospholipase D1/2) proteins, as well as members of the Ras signaling pathway. In addition, the proteome contained an unusually large proportion (53.6%) of hypothetical proteins, the majority of which (85.8%) were Glomeromycota-specific. Forty-eight proteins were predicted to be surface/membrane a...
Source: Fungal Biology - Category: Biology Source Type: research