Phototemtide A, a Cyclic Lipopeptide Heterologously Expressed from Photorhabdus temperata Meg1, Shows Selective Antiprotozoal Activity.

Phototemtide A, a Cyclic Lipopeptide Heterologously Expressed from Photorhabdus temperata Meg1, Shows Selective Antiprotozoal Activity.
Chembiochem. 2019 Dec 09;:
Authors: Zhao L, Vo TD, Kaiser M, Bode HB
Abstract
A new cyclic lipopeptide, phototemtide A (1), was isolated from Escherichia coli expressing the biosynthetic gene cluster pttABC from Photorhabdus temperata Meg1. The structure of 1 was elucidated by HR-ESI-MS and NMR experiments. The absolute configurations of amino acids and 3-hydroxyoctanoic acid in 1 were determined by using the advanced Marfey's method and comparison after total synthesis of 1, respectively. Additionally, three new minor derivatives, phototemtides B-D (2-4), were identified by detailed HPLC-MS analysis. Phototemtide A (1) showed weak antiprotozoal activity against Plasmodium falciparum, with an IC50 value of 9.8 μM. The biosynthesis of phototemtides A-D (1-4) was also proposed.
PMID: 31814269 [PubMed - as supplied by publisher]

Source: Chembiochem - December 8, 2019 Category: Biochemistry Authors: Zhao L, Vo TD, Kaiser M, Bode HB Tags: Chembiochem Source Type: research