Genome-wide analysis of mammary gland shows modulation of transcriptome landscape with alternative splice variants in Staphylococcus aureus mastitis in mice.

Genome-wide analysis of mammary gland shows modulation of transcriptome landscape with alternative splice variants in Staphylococcus aureus mastitis in mice. Gene. 2019 Dec 07;:144278 Authors: Mitra SD, Ganaie F, Bankar K, Velu D, Mani B, Vasudevan M, Shome R, Rahman H, Kumar Ghosh S, Shome BR Abstract Epidemiological mapping shows Staphylococcus aureus to be the leading mastitis causing pathogen in India with diverse genetic lineages circulating in the dairy cattle population. We previously reported that endemic clonal strains of S. aureus isolated from subclinical mastitis lead to specific alteration of epigenetic modulators resulting in deviating immune response in intramammary infection mouse model. However, the extent of transcriptome modulation and associated alternative splicing in S. aureus mastitis is poorly understood. Hence, to gain a deeper insight of the extent of modulation of transcriptome landscape, we expanded the study here using high throughput, paired-end RNA sequencing analysis of the mouse mammary gland inoculated with three strains of S. aureus (SA1, SA2, and SA3) possessing specific genotype, virulence and enterotoxin traits. Overall, we detected 35878 transcripts in S. aureus inoculated mammary gland, 23% more than those annotated in the reference genome. Expression of 20756 transcripts was >1 fragment per kilobase of transcript per million mapped fragments and 25.95% of multi-exonic genes were alternative...
Source: Gene - Category: Genetics & Stem Cells Authors: Tags: Gene Source Type: research