Cancers, Vol. 11, Pages 1990: How Valuable Is the RT-qPCR of Pituitary-Specific Transcription Factors for Identifying Pituitary Neuroendocrine Tumor Subtypes According to the New WHO 2017 Criteria?
Cancers, Vol. 11, Pages 1990: How Valuable Is the RT-qPCR of Pituitary-Specific Transcription Factors for Identifying Pituitary Neuroendocrine Tumor Subtypes According to the New WHO 2017 Criteria?
Cancers doi: 10.3390/cancers11121990
Authors:
María Eugenia Torregrosa-Quesada
Araceli García-Martínez
Sandra Silva-Ortega
Sebastián Martínez-López
Rosa Cámara
Carmen Fajardo
Cristina Lamas
Ignacio Aranda
Antonio Picó
The classification of pituitary neuroendocrine tumors (PitNETs) subtypes continues generating interest. In 2017, the World Health Organization (WHO) proposed considering the immunohistochemical (IHC) analysis of pituitary-specific transcription factors (TF) for their typification. The present study targeted the quantification of pituitary-specific TF (TPIT, PIT-1, SF-1, GATA2, ESR1) gene expression by RT-qPCR to overcome the shortcomings of IHC and to complement it. We analyzed 251 tumors from our collection of PitNETs and performed additional IHC studies in a subset of 56 samples to analyze the concordance between gene and protein expression of the TF. The molecular and IHC studies allowed us to significantly reduce the percentage of null cell tumors in our series, most of which were reclassified as gonadotroph tumors. The concordance between the molecular and the immunohistochemical studies was good for tumors coming from the corticotroph and Pit-1 lineages but worsened for the rest of the tumors. Indeed, the RT-qPCR helped to improve...
Source: Cancers - Category: Cancer & Oncology Authors: Mar ía Eugenia Torregrosa-Quesada Araceli Garc ía-Martínez Sandra Silva-Ortega Sebasti án Martínez-López Rosa C ámara Carmen Fajardo Cristina Lamas Ignacio Aranda Antonio Pic ó Tags: Article Source Type: research
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