Development of DNA vaccines encoding ribosomal proteins (RplL and RpsA) against Nocardia seriolae infection in fish

Publication date: Available online 9 December 2019Source: Fish &Shellfish ImmunologyAuthor(s): Jianlin Chen, Zhenwei Chen, Wenji Wang, Suying Hou, Jia Cai, Liqun Xia, Yishan LuAbstractNocardia seriolae, a Gram-positive pathogen, has been identified as the causative agent of fish nocardiosis. DNA vaccination has been proven to be effective in conferring protection against bacterial infection in fish. The 30S ribosomal protein S1 (RpsA) and 50S ribosomal protein L7/L12 (RplL) were identified to be the common immunodominant antigens of three fish pathogenic Nocardia (N. seriolae, N. salmonicida and N. asteroids) by immunoproteomics profiling in our previous study. In current study, the immunogenicity and protective efficacy of two DNA vaccines encoding RplL and RpsA were evaluated and compared in hybrid snakehead. The results showed vaccination of hybrid snakehead with the pcDNA-RplL and pcDNA-RpsA DNA vaccines provided protective efficacy with relative percentage survival (RPS) of 78.31% and 71.08%, respectively. Meanwhile, the immune response of hybrid snakehead induced by these two DNA vaccines were investigated, and it revealed that the non-specific immunity parameters (serum lysozyme (LYZ), peroxidase (POD), acid phosphatase (ACP), alkaline phosphatase (AKP) and superoxide dismutase (SOD) activities), specific antibody (IgM) production and immune-related genes expression (MHCIα, MHCIIα, CD4, CD8α, IL-1β and TNFα) were significantly increased...
Source: Fish and Shellfish Immunology - Category: Biology Source Type: research

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Source: Global Public Health - Category: International Medicine & Public Health Authors: Source Type: research
Publication date: Available online 21 January 2020Source: The Lancet Respiratory MedicineAuthor(s): Thomas C Williams, Sonnie Kim, David J Spiro, Harry Campbell
Source: The Lancet Respiratory Medicine - Category: Respiratory Medicine Source Type: research
Cases of a novel pneumonia-like illness that originated in Wuhan, China in December have now been confirmed in South Korea, Thailand, Japan, Taiwan, Macau—and, as of Jan. 21, the U.S. The virus’ geographic reach, as well as its newly discovered ability to spread via person-to-person contact, has health officials worried about the prospect of globals spread. As health officials scramble to learn more about the virus and is origins, researchers are simultaneously turning to the question of how to develop a vaccine or therapy that could help contain transmission worldwide—a feat that experts say is technical...
Source: TIME: Health - Category: Consumer Health News Authors: Tags: Uncategorized Infectious Disease Source Type: news
Have pediatric influenza vaccines resulted in a reduction in antibiotic utilization and resistance?Emerging Infectious Diseases
Source: Medscape Today Headlines - Category: Consumer Health News Tags: Infectious Diseases Journal Article Source Type: news
Condition:   Meningococcal Infections Intervention:   Biological: Meningococcal Group ACYW135 Conjugate Vaccine Sponsor:   Walvax Biotechnology Co., Ltd. Completed
Source: ClinicalTrials.gov - Category: Research Source Type: clinical trials
Conditions:   Shoulder Injury Related to Vaccine Administration;   Erosion, Localized Intervention:   Diagnostic Test: Ultrasound Shoulder and Immunological Phenotyping by FACS Sponsor:   University Hospital, Basel, Switzerland Completed
Source: ClinicalTrials.gov - Category: Research Source Type: clinical trials
BOSTON (CBS) – Kids are visiting their pediatricians less often than they did a decade ago. That’s according to a study published in JAMA Pediatrics. Researchers at the University of Pittsburgh found that between 2008 and 2016, “well child” visits increased nearly 10-percent, but that was overshadowed by a 24-percent drop in problem-based visits for illness and injury. Perhaps better preventative care is keeping kids healthier or providers are providing more support over the phone. But parents may also be avoiding the doctor’s office due to high co-pays and deductibles or may be taking their k...
Source: WBZ-TV - Breaking News, Weather and Sports for Boston, Worcester and New Hampshire - Category: Consumer Health News Authors: Tags: Boston News Health Healthwatch Syndicated CBSN Boston Syndicated Local Dr. Mallika Marshall Health News Source Type: news
You’ve done it! You’ve taken that last birth control pill, removed your IUD, or stopped using your contraceptive method of choice. You’ve made the decision to try to conceive a pregnancy, and while this is an exciting time in your life, it can also feel overwhelming. There is so much advice around fertility and pregnancy, and sifting through it all just isn’t possible. For many mothers, their goals crystallize around ensuring that their baby is healthy. Evidence-based steps that may prevent birth defects January is Birth Defects Prevention Month, so we want to focus on things you can do to reduce th...
Source: Harvard Health Blog - Category: Consumer Health News Authors: Tags: Family Planning and Pregnancy Fertility Vaccines Women's Health Source Type: blogs
Publication date: 21 January 2020Source: Cell Reports, Volume 30, Issue 3Author(s): Felix Horns, Cornelia L. Dekker, Stephen R. QuakeSummaryAntibody memory protects humans from many diseases. Protective antibody memory responses require activation of transcriptional programs, cell proliferation, and production of antigen-specific antibodies, but how these aspects of the response are coordinated is poorly understood. We profile the molecular and cellular features of the antibody response to influenza vaccination by integrating single-cell transcriptomics, longitudinal antibody repertoire sequencing, and antibody binding mea...
Source: Cell Reports - Category: Cytology Source Type: research
CONCLUSION We identified a population of HCV-specific CD4+ T cells with a follicular T helper cell signature that is maintained after therapy-induced elimination of persistent infection and may constitute an important target population for vaccination efforts to prevent reinfection and immunotherapeutic approaches for persistent viral infections.FUNDING Deutsche Forschungsgemeinschaft (DFG, German Research Foundation), the National Institute of Allergy and Infectious Diseases (NIAID), the European Union, the Berta-Ottenstein-Programme for Advanced Clinician Scientists, and the ANRS.
Source: Journal of Clinical Investigation - Category: Biomedical Science Authors: Source Type: research
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