Suppression of time-dependent decay by controlling the redox balance of human induced pluripotent stem cells suspended in a cryopreservation solution

In this study, the aim was to investigate a critical factor of cell decay in human induced pluripotent stem cells suspended in a cryopreservation solution and to propose a methodology to maintain the viability of cells for a longer filling process time. Based on experimental results, it was determined that the accumulation of intracellular reactive oxygen species induces apoptosis of cells suspended in a cryopreservation solution. In addition, treatment with an antioxidant or a low-temperature condition was effective in suppressing the induction of apoptosis and subsequent cell death. In particular, the treatment at low temperature was able to maintain the attachment efficiency of suspended cells at a higher level compared to the treatment with antioxidants. The appropriate control of redox balance in cells suspended in a cryopreservation solution could improve the viability of cells in the filling process and lead to the successful lot size expansion of cell manufacturing systems.
Source: Biochemical Engineering Journal - Category: Biochemistry Source Type: research