Transcriptomic profile of chicken Bone Marrow-Derive Dendritic cells in response to H9N2 avian influenza A virus

Publication date: Available online 9 December 2019Source: Veterinary Immunology and ImmunopathologyAuthor(s): Qingtao Liu, Jing Yang, Xinmei Huang, Yuzhuo Liu, Kaikai Han, Dongmin Zhao, Lijiao Zhang, Yin LiAbstractAvian influenza subtype H9N2 infection is a mild but highly contagious disease that is associated with a decrease in the efficacy of vaccine interventions, and an increase in susceptibility to secondary infections in poultry. However, the immune evasion mechanism of H9N2 avian influenza viruses (AIVs) in chickens is poorly understood. Dendritic cells (DCs) are immune cells of major importance, involved in innate immune responses against viruses, but also in the setting of adaptive immune response due to their high ability to present viral antigen. Therefore, in the present study we used high-throughput RNA-sequencing technology at the transcriptome level to identify the differentially expressed genes (DEGs) between chicken DCs infected with H9N2 virus and mock-infected DCs. We identified 4151 upregulated DEGs and 2138 downregulated DEGs. Further enrichment analysis showed that the upregulated DEGs were enriched in the biological processes mainly involved in signal transduction, transmembrane transport, and innate immune/inflammatory responses. In contrast, the downregulated DEGs were associated with the biological processes mainly including metabolic process, and MHC class I antigen processing and presentation. In addition, 49 of these immune-related DEGs were valid...
Source: Veterinary Immunology and Immunopathology - Category: Veterinary Research Source Type: research